ArticleNucleic acids research2010
Structure of an archaeal non-discriminating glutamyl-tRNA synthetase: a missing link in the evolution of Gln-tRNAGln formation.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Architecture ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- AARS Online: A collaborative database on the structure, function, and evolution of the aminoacyl-tRNA synthetases.IUBMB life · 2024Article
- Enzymic recognition of amino acids drove the evolution of primordial genetic codes.Nucleic acids research · 2024Article
- Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1.Nature communications · 2022Article
- On the Track of the Missing tRNA Genes: A Source of Non-Canonical Functions?Frontiers in molecular biosciences · 2021Review
- Evolution of Life on Earth: tRNA, Aminoacyl-tRNA Synthetases and the Genetic Code.Life (Basel, Switzerland) · 2020Review
- PhyloToL: A Taxon/Gene-Rich Phylogenomic Pipeline to Explore Genome Evolution of Diverse Eukaryotes.Molecular biology and evolution · 2019Article
- Quest for Ancestors of Eukaryal Cells Based on Phylogenetic Analyses of Aminoacyl-tRNA Synthetases.Journal of molecular evolution · 2017Article
- A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes.Genome research · 2016Article
- The crystal structure of human GlnRS provides basis for the development of neurological disorders.Nucleic acids research · 2016Article
- Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.The Journal of biological chemistry · 2015Article
- Dispensability of zinc and the putative zinc-binding domain in bacterial glutamyl-tRNA synthetase.Bioscience reports · 2015Article
- Expanded microbial genome coverage and improved protein family annotation in the COG database.Nucleic acids research · 2015Article
- Gene rearrangements in gekkonid mitochondrial genomes with shuffling, loss, and reassignment of tRNA genes.BMC genomics · 2014Article
- Evolutionary insights about bacterial GlxRS from whole genome analyses: is GluRS2 a chimera?BMC evolutionary biology · 2014Article
- Emergence and evolution.Topics in current chemistry · 2014Review
- The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.Journal of molecular biology · 2013Article
- Rational design and directed evolution of a bacterial-type glutaminyl-tRNA synthetase precursor.Nucleic acids research · 2012Article
- Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis.The Journal of biological chemistry · 2012Article
- Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.Nucleic acids research · 2012Article
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9 authors at 1 institution in 1 country.
Funding
Abstract
The molecular basis of the genetic code relies on the specific ligation of amino acids to their cognate tRNA molecules. However, two pathways exist for the formation of Gln-tRNA(Gln). The evolutionarily older indirect route utilizes a non-discriminating glutamyl-tRNA synthetase (ND-GluRS) that can form both Glu-tRNA(Glu) and Glu-tRNA(Gln). The Glu-tRNA(Gln) is then converted to Gln-tRNA(Gln) by an amidotransferase. Since the well-characterized bacterial ND-GluRS enzymes recognize tRNA(Glu) and tRNA(Gln) with an unrelated α-helical cage domain in contrast to the β-barrel anticodon-binding domain in archaeal and eukaryotic GluRSs, the mode of tRNA(Glu)/tRNA(Gln) discrimination in archaea and eukaryotes was unknown. Here, we present the crystal structure of the Methanothermobacter thermautotrophicus ND-GluRS, which is the evolutionary predecessor of both the glutaminyl-tRNA synthetase (GlnRS) and the eukaryotic discriminating GluRS. Comparison with the previously solved structure of the Escherichia coli GlnRS-tRNA(Gln) complex reveals the structural determinants responsible for specific tRNA(Gln) recognition by GlnRS compared to promiscuous recognition of both tRNAs by the ND-GluRS. The structure also shows the amino acid recognition pocket of GluRS is more variable than that found in GlnRS. Phylogenetic analysis is used to reconstruct the key events in the evolution from indirect to direct genetic encoding of glutamine.
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