ArticleApplied and environmental microbiology2010
Identification of compounds with bioactivity against the nematode Caenorhabditis elegans by a screen based on the functional genomics of the marine bacterium Pseudoalteromonas tunicata D2.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Multi-omics analysis of the correlation between surface microbiome and metabolome in Saccharina latissima (Laminariales, Phaeophyceae).FEMS microbiology ecology · 2025Article
- Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host-Fungal Interactions.Journal of fungi (Basel, Switzerland) · 2023Review
- Impact of Marine Chemical Ecology Research on the Discovery and Development of New Pharmaceuticals.Marine drugs · 2023Review
- Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae.Microorganisms · 2022Article
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- Specialized Metabolite-Mediated Predation Defense in the Marine ActinobacteriumApplied and environmental microbiology · 2022Article
- Novel Nematode-Killing Protein-1 (Nkp-1) from a Marine Epiphytic BacteriumBiomedicines · 2021Article
- Development of Marine-Derived Compounds for Cancer Therapy.Marine drugs · 2021Review
- Review
- Combating Parasitic Nematode Infections, Newly Discovered Antinematode Compounds from Marine Epiphytic Bacteria.Microorganisms · 2020Review
- Slr4, a newly identified S-layer protein from marine Gammaproteobacteria, is a major biofilm matrix component.Molecular microbiology · 2020Article
- Similar bacterial communities on healthy and injured skin of black tip reef sharks.Animal microbiome · 2019Article
- Article
- Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria,Frontiers in microbiology · 2019Review
- Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas.International journal of molecular sciences · 2018Article
- Summer shifts of bacterial communities associated with the invasive brown seaweed Sargassum muticum are location and tissue dependent.PloS one · 2018Article
- Biofilm Formation and Heat Stress Induce Pyomelanin Production in Deep-SeaFrontiers in microbiology · 2017Article
- Improving Production of Protease from Pseudoalteromonas sp. CSN423 by Random Mutagenesis.Marine biotechnology (New York, N.Y.) · 2016Article
- Caenorhabditis elegans employs innate and learned aversion in response to bacterial toxic metabolites tambjamine and violacein.Scientific reports · 2016Article
- Genetically and Phenotypically Distinct Pseudomonas aeruginosa Cystic Fibrosis Isolates Share a Core Proteomic Signature.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine bacteria are a rich, yet underexplored, resource of compounds with inhibitory bioactivity against a range of eukaryotic target organisms. Identification of those inhibitors, however, requires a culturable or genetically tractable producer strain, a prerequisite that is not often fulfilled. This study describes a novel functional genomic screen that is based on expression of inhibitors in a heterogeneous recombinant host (i.e., Escherichia coli). Functional libraries were screened by selective grazing by the nematode Caenorhabditis elegans, in a simple, rapid, high-throughput manner. We applied our approach to discover inhibitors of C. elegans produced by the marine bacterium Pseudoalteromonas tunicata D2, a model organism for exploring a range of antagonistic activities between bacteria and eukaryotes and a known producer of several toxic compounds. Expression of P. tunicata DNA in E. coli and grazing selection by the nematode Caenorhabditis elegans identified two clones, with slow- and fast-killing modes of action. Genomic analysis of the slow-killing clone revealed that the activity was due to a small molecule, tambjamine, while the fast-killing activity involved a gene encoding for a novel protein. Microscopic analysis showed substantial colonization of the intestinal lumen, or rapid death of the nematode without colonization, for the two activities, respectively. The novel functional genomic screen presented here therefore detects new eukaryotic inhibitors with different chemical structures, kinetics, and predicted modes of actions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.