ArticleJournal of bacteriology2010
DNA uptake sequence-mediated enhancement of transformation in Neisseria gonorrhoeae is strain dependent.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- The role of extracellular DNA inJournal of bacteriology · 2026Review
- Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species.Applied microbiology and biotechnology · 2026Article
- Fluoroquinolone resistance-conferringbioRxiv : the preprint server for biology · 2026Article
- The Transformation Experiment of Frederick Griffith II: Inclusion of Cellular Heredity for the Creation of Novel Microorganisms.Bioengineering (Basel, Switzerland) · 2025Review
- Discovery of the widespread site-specific single-stranded nuclease family Ssn.Nature communications · 2025Article
- Novobiocin primarily targets ParE in Neisseria gonorrhoeae.The Journal of antibiotics · 2025Article
- Carriage and antimicrobial susceptibility of commensal Neisseria species from the human oropharynx.Scientific reports · 2024Article
- Genetic Manipulation of Neisseria gonorrhoeae and Commensal Neisseria Species.Current protocols · 2024Article
- Development and implementation of a Type I-C CRISPR-based programmable repression system formBio · 2024Article
- Bacterium of one thousand and one variants: genetic diversity ofMicrobial genomics · 2023Review
- Multisite transformation inFrontiers in microbiology · 2023Article
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2022Article
- Successful Intra- but Not Inter-species Recombination ofFrontiers in microbiology · 2022Article
- Antimicrobial Resistance Profiles of Human CommensalAntibiotics (Basel, Switzerland) · 2021Article
- Evidence of Horizontal Gene Transfer of 50S Ribosomal GenesFrontiers in microbiology · 2021Article
- Article
- Commensal Neisseria Kill Neisseria gonorrhoeae through a DNA-Dependent Mechanism.Cell host & microbe · 2019Article
- Article
- Progress Toward a Gonococcal Vaccine: The Way Forward.Frontiers in immunology · 2019Review
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2 authors.
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Abstract
Natural transformation is the main means of horizontal genetic exchange in the obligate human pathogen Neisseria gonorrhoeae. Neisseria spp. have been shown to preferentially take up and transform their own DNA by recognizing the nonpalindromic 10- or 12-nucleotide sequence 5'-ATGCCGTCTGAA-3' (additional semiconserved nucleotides are underlined), termed the DNA uptake sequence (DUS10 or DUS12). Here we investigated the effects of the DUS on transformation and DNA uptake for several laboratory strains of N. gonorrhoeae. We found that all strains showed efficient transformation of DUS containing DNA (DUS10 and DUS12) but that the level of transformation with DNA lacking a DUS (DUS0) was variable in different strains. The DUS-enhanced transformation was 20-fold in two strains, FA1090 and FA19, but was approximately 150-fold in strains MS11 and 1291. All strains tested provide some level of DUS0 transformation, and DUS0 transformation was type IV pilus dependent. Competition with plasmid DNA revealed that transformation of MS11 was enhanced by the addition of excess plasmid DNA containing a DUS while FA1090 transformation was competitively inhibited. Although FA1090 was able to mediate much more efficient transformation of DNA lacking a DUS than was MS11, DNA uptake experiments showed similar levels of uptake of DNA containing and lacking a DUS in FA1090 and MS11. Finally, DNA uptake was competitively inhibited in both FA1090 and MS11. Taken together, our data indicate that the role of the DUS during DNA transformation is variable between strains of N. gonorrhoeae and may influence multiple steps during transformation.
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