ArticleAmerican journal of physiology. Heart and circulatory physiology2010
High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS.
Article in American journal of physiology. Heart and circulatory physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- A large population of cell-specific action potential models replicating fluorescence recordings of voltage in rabbit ventricular myocytes.Royal Society open science · 2025Article
- Active Micro-Nano-Collaborative Bioelectronic Device for Advanced Electrophysiological Recording.Nano-micro letters · 2024Review
- The calcium transient coupled to the L-type calcium current attenuates cardiac alternans.Frontiers in physiology · 2024Article
- Electrophysiological heterogeneity in large populations of rabbit ventricular cardiomyocytes.Cardiovascular research · 2022Article
- Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.Progress in biophysics and molecular biology · 2020Review
- METROID: an automated method for robust quantification of subcellular fluorescence events at low SNR.BMC bioinformatics · 2020Article
- Regulation of CaCell calcium · 2019Article
- Examination of the Effects of Conduction Slowing on the Upstroke of Optically Recorded Action Potentials.Frontiers in physiology · 2019Article
- Blockade of CaMKII depresses conduction preferentially in the right ventricular outflow tract and promotes ischemic ventricular fibrillation in the rabbit heart.American journal of physiology. Heart and circulatory physiology · 2017Article
- Evaluation of excitation propagation in the rabbit heart: optical mapping and transmural microelectrode recordings.PloS one · 2015Article
- A near-infrared fluorescent voltage-sensitive dye allows for moderate-throughput electrophysiological analyses of human induced pluripotent stem cell-derived cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2014Article
- Caveolae in ventricular myocytes are required for stretch-dependent conduction slowing.Journal of molecular and cellular cardiology · 2014Article
- Rapid cellular phenotyping of human pluripotent stem cell-derived cardiomyocytes using a genetically encoded fluorescent voltage sensor.Stem cell reports · 2014Article
- Fibroblast growth factor homologous factors modulate cardiac calcium channels.Circulation research · 2013Article
- Article
- Optical mapping at increased illumination intensities.Journal of biomedical optics · 2012Article
- Optical imaging of voltage and calcium in cardiac cells & tissues.Circulation research · 2012Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
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Abstract
The use of voltage-sensitive fluorescent dyes (VSD) for noninvasive measurement of the action potential (AP) in isolated cells has been hindered by low-photon yield of the preparation, dye toxicity, and photodynamic damage. Here we used a new red-shifted VSD, di-4-ANBDQBS, and a fast electron-multiplied charge-coupled device camera for optical AP (OAP) recording in guinea pig cardiac myocytes. Loading di-4-ANBDQBS did not alter APs recorded with micropipette. With short laser exposures (just enough to record one OAP every 1-5 min), di-4-ANBDQBS yielded fluorescent signals with very high signal-to-background ratios (change in fluorescence on depolarization/fluorescence at resting potential: 19.2 ± 4.1%) and signal-to-noise ratios (40 ± 13.2). Quantum chemical calculations comparing the ANBDQ chromophore to the conventional ANEP chromophore showed that the higher wavelength and the greater voltage sensitivity of the former have the same electro-optical origin: a longer path for electron redistribution in the excited state. OAP closely tracked simultaneously recorded electrical APs, permitting measurement of AP duration within 1% error. Prolonged laser exposure caused progressive AP duration prolongation and instability. However, these effects were alleviated or abolished by reducing the dye concentration and by perfusion with antioxidants. Thus the presented technique provides a unique opportunity for noninvasive AP recording in single cardiomyocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.