ArticleJournal of immunological methods2010
Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity.
Article in Journal of immunological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Resolving inflammation: The impact of antiretroviral therapy on macrophage traffic in and out of the CNS.PLoS pathogens · 2025Article
- Cellular immunophenotyping in human and primate tissues during healthy conditions and Ebola and Nipah infections.JCI insight · 2025Article
- Cellular immune changes during severe antisense oligonucleotide-associated thrombocytopenia in a nonhuman primate model.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Design of a Robust Flow Cytometric Approach for Phenotypical and Functional Analysis of Human Monocyte Subsets in Health and Disease.Biomolecules · 2024Article
- The TLR2/TLR6 ligand FSL-1 mitigates radiation-induced hematopoietic injury in mice and nonhuman primates.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An ex vivo model of interactions between extracellular vesicles and peripheral mononuclear blood cells in whole blood.Journal of extracellular vesicles · 2023Article
- Innate cell markers that predict anti-HIV neutralizing antibody titers in vaccinated macaques.Cell reports. Medicine · 2022Article
- A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates.Frontiers in immunology · 2022Article
- Immunological Feature and Transcriptional Signaling of Ly6C Monocyte Subsets From Transcriptome Analysis in Control and Hyperhomocysteinemic Mice.Frontiers in immunology · 2021Article
- Monocytopenia, monocyte morphological anomalies and hyperinflammation characterise severe COVID-19 in type 2 diabetes.EMBO molecular medicine · 2020Observational
- Late effects of total body irradiation on hematopoietic recovery and immune function in rhesus macaques.PloS one · 2019Article
- A pilot study evaluating the utility of commercially available antibodies for flow cytometric analysis of Panthera species lymphocytes.BMC veterinary research · 2018Article
- Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis.Journal of visualized experiments : JoVE · 2018Article
- Novel Strategy To Adapt Simian-Human Immunodeficiency Virus E1 CarryingJournal of virology · 2018Article
- HIV vaccine candidate activation of hypoxia and the inflammasome in CD14Nature medicine · 2018Article
- Prime and Boost Vaccination Elicit a Distinct Innate Myeloid Cell Immune Response.Scientific reports · 2018Article
- Infection with the Makona variant results in a delayed and distinct host immune response compared to previous Ebola virus variants.Scientific reports · 2017Article
- Toll-like receptor 2Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
- Divergent Immunomodulation Capacity of Individual Myelin Peptides-Components of Liposomal Therapeutic against Multiple Sclerosis.Frontiers in immunology · 2017Article
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4 authors.
Funding
Abstract
Monitoring changes in rhesus macaque immune cell populations during infectious disease is crucial. The aim of this work was to simultaneously analyze the phenotype of rhesus macaque lymphocyte, monocyte and dendritic cell (DC) subsets using a single 12-color flow cytometry panel. Blood from healthy non-infected rhesus macaques was labeled with a cocktail of 12 antibodies. Data were compared to three smaller lineage specific panels and absolute and relative percentages of cells were compared. Our 12-color panel allows for the identification of the following major subsets: CD4+ and CD8+ T lymphocytes, B lymphocytes, natural killer (NK) cells, natural killer T (NKT) cells, monocyte subsets and four non-overlapping Lin-HLA-DR+ cell subsets: CD34+ hematopoietic stem cells, CD11c- CD123+ plasmacytoid DC, CD11c+ CD16+ and CD11c(-)(/dim) CD1c+ myeloid DC. The development of a multiparameter flow cytometry panel will allow for simultaneous enumeration of mature lymphocyte, NK cells, monocyte and DC subsets. Studying these major players of the immune system in one panel may give us a broader view of the immune response during SIV infection and the ability to better define the role of each of these individual cell types in the pathogenesis of AIDS.
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