ArticleLab on a chip2010
Digital PCR on a SlipChip.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
89 citing papers in PubMed.
- PEG-mediated viscous crowding controls dynamics and quantitative range in recombinase polymerase amplification nucleation site analysis.Sensors & diagnostics · 2026Article
- Advances of Digital Detection for Foodborne Pathogens.Foods (Basel, Switzerland) · 2026Review
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- Rapid Isothermal DNA Amplification in Microchambers Detected by Fluorescence RNA Aptamer Transcription.Diagnostics (Basel, Switzerland) · 2025Article
- High-Density Microfluidic Chip with Vertical Structure for Digital PCR.Sensors (Basel, Switzerland) · 2025Article
- Hydrophilic/Omniphobic Droplet Arrays for High-throughput and Quantitative Enzymology.Analytical chemistry · 2025Article
- PDMS SlipChip: Optimizing Sealing, Slipping, and Biocompatibility Using Low-Viscosity Silicone Oils.Micromachines · 2025Article
- A Single-Cell Metabolic Profiling Characterizes Human Aging via SlipChip-SERS.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- All-In-One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Analysis of urine cell-free DNA in bladder cancer diagnosis by emerging bioactive technologies and materials.Frontiers in bioengineering and biotechnology · 2024Review
- SPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testing.Microsystems & nanoengineering · 2024Article
- Robust and rapid partitioning in thermoplastic.The Analyst · 2023Article
- Quantitative analysis of respiratory viruses based on lab-on-a-chip platform.Analytical and bioanalytical chemistry · 2023Article
- Virtual Multiplexing Chamber-Based Digital PCR for Camel Milk Authentication Applications.Micromachines · 2023Article
- Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies.Advanced healthcare materials · 2023Review
- Review
- Digital CRISPR Systems for the Next Generation of Nucleic Acid Quantification.Trends in analytical chemistry : TRAC · 2023Article
- Near-digital amplification in paper improves sensitivity and speed in biplexed reactions.Scientific reports · 2022Article
- Recent Advances in Digital Biosensing Technology.Biosensors · 2022Review
- Visible-light and near-infrared fluorescence and surface-enhanced Raman scattering point-of-care sensing and bio-imaging: a review.Chemical Society reviews · 2022Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
This paper describes a SlipChip to perform digital PCR in a very simple and inexpensive format. The fluidic path for introducing the sample combined with the PCR mixture was formed using elongated wells in the two plates of the SlipChip designed to overlap during sample loading. This fluidic path was broken up by simple slipping of the two plates that removed the overlap among wells and brought each well in contact with a reservoir preloaded with oil to generate 1280 reaction compartments (2.6 nL each) simultaneously. After thermal cycling, end-point fluorescence intensity was used to detect the presence of nucleic acid. Digital PCR on the SlipChip was tested quantitatively by using Staphylococcus aureus genomic DNA. As the concentration of the template DNA in the reaction mixture was diluted, the fraction of positive wells decreased as expected from the statistical analysis. No cross-contamination was observed during the experiments. At the extremes of the dynamic range of digital PCR the standard confidence interval determined using a normal approximation of the binomial distribution is not satisfactory. Therefore, statistical analysis based on the score method was used to establish these confidence intervals. The SlipChip provides a simple strategy to count nucleic acids by using PCR. It may find applications in research applications such as single cell analysis, prenatal diagnostics, and point-of-care diagnostics. SlipChip would become valuable for diagnostics, including applications in resource-limited areas after integration with isothermal nucleic acid amplification technologies and visual readout.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.