ArticlePloS one2010
Environmental exposure of the mouse germ line: DNA adducts in spermatozoa and formation of de novo mutations during spermatogenesis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 49 citations in OpenAlex.
- Mutation dynamics of CpG dinucleotides during a recent event of vertebrate diversification.Epigenetics · 2019Article
- Paternal use of antidepressants and offspring outcomes in Sweden: nationwide prospective cohort study.BMJ (Clinical research ed.) · 2018Observational
- Evaluation of in vivo mutagenesis for assessing the health risk of air pollutants.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2017Review
- Next generation sequencing of benzo(a)pyrene-induced lacZ mutants identifies a germ cell-specific mutation spectrum.Scientific reports · 2016Article
- Benzo(a)pyrene Is Mutagenic in Mouse Spermatogonial Stem Cells and Dividing Spermatogonia.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Sperm DNA oxidative damage and DNA adducts.Mutation research. Genetic toxicology and environmental mutagenesis · 2015Article
- Single cell gel electrophoresis (SCGE) and Pig-a mutation assay in vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo[a]pyrene in Ogg1(-/-) mice.Mutation research. Genetic toxicology and environmental mutagenesis · 2014Article
- Environmentally induced epigenetic transgenerational inheritance of male infertility.Current opinion in genetics & development · 2014Review
- Article
- Early-life exposure to benzo[a]pyrene increases mutant frequency in spermatogenic cells in adulthood.PloS one · 2014Article
- Paternal lifestyle as a potential source of germline mutations transmitted to offspring.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Paternal benzo[a]pyrene exposure affects gene expression in the early developing mouse embryo.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpermatozoal DNA damage is associated with poor sperm quality, disturbed embryonic development and early embryonic loss, and some genetic diseases originate from paternal de novo mutations. We previously reported poor repair of bulky DNA-lesions in rodent testicular cells. METHODOLOGY/PRINCIPAL
findingsWe studied the fate of DNA lesions in the male germ line. B[a]PDE-N(2)-dG adducts were determined by liquid chromatography-tandem mass spectrometry, and de novo mutations were measured in the cII-transgene, in Big Blue mice exposed to benzo[a]pyrene (B[a]P; 3 x 50 mg/kg bw, i.p.). Spermatozoa were harvested at various time-points following exposure, to study the consequences of exposure during the different stages of spermatogenesis. B[a]PDE-N(2)-dG adducts induced by exposure of spermatocytes or later stages of spermatogenesis persisted at high levels in the resulting spermatozoa. Spermatozoa originating from exposed spermatogonia did not contain DNA adducts; however de novo mutations had been induced (p = 0.029), specifically GC-TA transversions, characteristic of B[a]P mutagenesis. Moreover, a specific spectrum of spontaneous mutations was consistently observed in spermatozoa. CONCLUSIONS/SIGNIFICANCE: A temporal pattern of genotoxic consequences following exposure was identified, with an initial increase in DNA adduct levels in spermatozoa, believed to influence fertility, followed by induction of germ line de novo mutations with possible consequences for the offspring.
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