ArticleNature protocols2010
Fluorescent conjugated polymer-based FRET technique for detection of DNA methylation of cancer cells.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 100 citations in OpenAlex.
- Advances and Innovations in Conjugated Polymer Fluorescent Sensors for Environmental and Biological Detection.Biosensors · 2025Review
- Current status and new experimental diagnostic methods of invasive fungal infections after hematopoietic stem cell transplantation.Archives of microbiology · 2024Review
- Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review.Cancers · 2023Review
- Updates in Laboratory Identification of Invasive Fungal Infection in Neonates.Microorganisms · 2023Review
- A Label-Free, Mix-and-Detect ssDNA-Binding Assay Based on Cationic Conjugated Polymers.Biosensors · 2023Article
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- Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications.Polymers · 2020Review
- Efficient synthesis of 3,6,13,16-tetrasubstituted-tetrabenzo[Beilstein journal of organic chemistry · 2020Article
- A novel fluorescent biosensor based on dendritic DNA nanostructure in combination with ligase reaction for ultrasensitive detection of DNA methylation.Journal of nanobiotechnology · 2019Article
- Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy.Chemical science · 2017Article
- Article
- The Combination of Long-term Ketamine and Extinction Training Contributes to Fear Erasure by Bdnf Methylation.Frontiers in cellular neuroscience · 2017Article
- Technical advances in global DNA methylation analysis in human cancers.Journal of biological engineering · 2017Review
- Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays.Chemical science · 2015Article
- Convenient, sensitive and high-throughput method for screening botanic origin.Scientific reports · 2014Article
- Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt.Nature protocols · 2013Article
- Formation and characterization of stable fluorescent complexes between neutral conjugated polymers and cyclodextrins.Journal of fluorescence · 2013Article
- DNA methylation analysis in human cancer.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Detection and differential diagnosis of colon cancer by a cumulative analysis of promoter methylation.Nature communications · 2012Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes a homogeneous, convenient and sensitive DNA methylation detection method, using an optically amplifying cationic conjugated polymer (CCP, poly((1,4-phenylene)-2,7-[9,9-bis(6'-N,N,N-trimethyl ammonium)-hexyl fluorene] dibromide)). Genomic DNA from cancer cells is pretreated with a methylation-sensitive restriction endonuclease, followed by PCR amplification in the presence of fluorescein-labeled dNTP and Taq polymerase. The PCR only occurs for methylated DNA. DNA methylation of the gene sequence of interest is detected as a result of the fluorescence resonance energy transfer (FRET) between CCP and fluorescein that is incorporated into DNA. The methylated statuses of the p16, HPP1 and GALR2 promoters of five cancer cell lines (HT29, HepG2, A498, HL60 and M17) were assayed to provide an association study between the cancers and susceptibility genes, which shows great potential for early cancer diagnosis. This protocol simplifies previously available procedures by avoiding the need for primer labeling, isolation or purification steps, and sophisticated instruments. The assay takes about 20 h to obtain the methylated statuses of cancer cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.