ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2010
Development of real-time PCR assay for specific detection of cowpox virus.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Development and clinical validation of a colorimetric isothermal assay for rapid detection of monkeypox virus.New microbes and new infections · 2026Article
- Zoonoses in dog and cat shelters in North-East Italy: update on emerging, neglected and known zoonotic agents.Frontiers in veterinary science · 2024Article
- Poxviruses in Children.Advances in experimental medicine and biology · 2024Review
- The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus.BMC veterinary research · 2023Article
- Cowpox Viruses: A Zoo Full of Viral Diversity and Lurking Threats.Biomolecules · 2023Review
- Review
- Skin lesions caused byPostepy dermatologii i alergologii · 2020Article
- Seasonal recurrence of cowpox virus outbreaks in captive cheetahs (Acinonyx jubatus).PloS one · 2017Article
- Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay.Journal of virological methods · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe number of recorded human cowpox cases are recently increasing. The symptoms caused by cowpox virus (CPXV) in a number of human cases are close to the symptoms characteristic of the orthopoxviral human infections caused by monkeypox or smallpox (variola) viruses. Any rapid and reliable real-time PCR method for distinguishing cowpox from smallpox and monkeypox is yet absent.
objectivesThe aim of this study was to develop a quick and reliable real-time TaqMan PCR assay for specific detection of cowpox virus and to determine the sensitivity and specificity of this method. STUDY
designBased on aligned nucleotide sequences of orthopoxviruses, we found a virus-specific region in the CPXV genome and selected the oligonucleotide primers and hybridization probe within this region. The specificity of the developed method was tested using a panel of various orthopoxvirus (OPV) DNAs. The sensitivity was determined using the recombinant plasmid carrying a fragment of CPXV DNA and genomic DNA of the CPXV strain GRI-90.
resultsThe analytical specificity of this method was determined using DNAs of 17 strains of four OPV species pathogenic for humans and amounted to 100%. The method allows 6 copies of plasmid DNA and 20 copies of CPXV DNA in the reaction mixture to be detected.
conclusionA quick and reliable TaqMan PCR assay providing for a highly sensitive and specific detection of CPXV DNA was developed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.