ArticleBMC plant biology2010
Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 66 citations in OpenAlex.
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- Characterization of Non-heading Mutation in Heading Chinese Cabbage (Frontiers in plant science · 2019Article
- Dissection of the genetic architecture of three seed-quality traits and consequences for breeding in Brassica napus.Plant biotechnology journal · 2018Article
- Integrated QTL and eQTL Mapping Provides Insights and Candidate Genes for Fatty Acid Composition, Flowering Time, and Growth Traits in a FFrontiers in plant science · 2018Article
- Development and validation of functional CAPS markers for the FAE genes inBreeding science · 2016Article
- Characteristics Analysis of F1 Hybrids between Genetically Modified Brassica napus and B. rapa.PloS one · 2016Article
- Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.PloS one · 2016Article
- EcoTILLING-Based Association Mapping Efficiently Delineates Functionally Relevant Natural Allelic Variants of Candidate Genes Governing Agronomic Traits in Chickpea.Frontiers in plant science · 2016Article
- Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.Plant physiology · 2015Article
- Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.Breeding science · 2015Article
- State of the science and challenges of breeding landscape plants with ecological function.Horticulture research · 2015Review
- Genome-wide investigation of genetic changes during modern breeding of Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFAE1 (fatty acid elongase1) is the key gene in the control of erucic acid synthesis in seeds of Brassica species. Due to oil with low erucic acid (LEA) content is essential for human health and not enough LEA resource could be available, thus new LEA genetic resources are being sought for Brassica breeding. EcoTILLING, a powerful genotyping method, can readily be used to identify polymorphisms in Brassica.
resultsSeven B. rapa, nine B. oleracea and 101 B. napus accessions were collected for identification of FAE1 polymorphisms. Three polymorphisms were detected in the two FAE1 paralogues of B. napus using EcoTILLING and were found to be strongly associated with differences in the erucic acid contents of seeds. In genomic FAE1 sequences obtained from seven B. rapa accessions, one SNP in the coding region was deduced to cause loss of gene function. Molecular evolution analysis of FAE1 homologues showed that the relationship between the Brassica A and C genomes is closer than that between the A/C genomes and Arabidopsis genome. Alignment of the coding sequences of these FAE1 homologues indicated that 18 SNPs differed between the A and C genomes and could be used as genome-specific markers in Brassica.
conclusionThis study showed the applicability of EcoTILLING for detecting gene polymorphisms in Brassica. The association between B. napus FAE1 polymorphisms and the erucic acid contents of seeds may provide useful guidance for LEA breeding. The discovery of the LEA resource in B. rapa can be exploited in Brasscia cultivation.
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