ReviewBiopolymers2010
Peptide reporters of kinase activity in whole cell lysates.
Review in Biopolymers, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Subtimizer: Computational Workflow for Structure-Guided Design of Potent and Selective Kinase Peptide Substrates.Journal of chemical information and modeling · 2026Article
- Analysis of Kinase-Peptide Interactions: DGMM Latent Constructs and Clustering Towards New Groups and Stand-Out Structures.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.ACS measurement science au · 2024Article
- LIMS-Kinase provides sensitive and generalizable label-freeCell reports. Physical science · 2023Article
- Review
- Kinome-wide analysis of the effect of statins in colorectal cancer.British journal of cancer · 2021Article
- A Novel ELISA-Based Peptide Biosensor Assay for Screening ABL1 ActivityFrontiers in pharmacology · 2021Article
- Assays for tyrosine phosphorylation in human cells.Methods in enzymology · 2019Article
- Sensing Enzymatic Activity by Exposure and Selection of DNA-Encoded Probes.Angewandte Chemie (International ed. in English) · 2016Article
- Fluorescence-based bioassays for the detection and evaluation of food materials.Sensors (Basel, Switzerland) · 2015Review
- A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity.Chemical communications (Cambridge, England) · 2015Article
- Long-wavelength fluorescent reporters for monitoring protein kinase activity.Angewandte Chemie (International ed. in English) · 2014Article
- Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells.Angewandte Chemie (International ed. in English) · 2013Article
- Activity assay of epidermal growth factor receptor tyrosine kinase inhibitors in triple-negative breast cancer cells using peptide-conjugated magnetic beads.Assay and drug development technologies · 2013Article
- Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS.Molecular bioSystems · 2012Article
- Development of a peptidase-resistant substrate for single-cell measurement of protein kinase B activation.Analytical chemistry · 2012Article
- Metabolism of peptide reporters in cell lysates and single cells.The Analyst · 2012Article
- Major remodelling of the murine stem cell kinome following differentiation in the hematopoietic compartment.Journal of proteome research · 2011Article
- A magnetic bead-based protein kinase assay with dual detection techniques.Analytical biochemistry · 2011Article
- Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS.Bioconjugate chemistry · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Kinase assays are used to screen for small-molecule inhibitors that may show promise as targeted pharmaceutical therapies. Using cell lysates instead of purified kinases provides a more accurate estimate of inhibitor sensitivity and selectivity in a biological setting. This review summarizes the range of homogeneous (solution-phase) and heterogeneous (solid-supported) formats available for using peptide substrates to monitor kinase activities in cell lysates. With a focus on heterogeneous kinase assays, the peptide substrate Abltide is used as a model to optimize presentation geometries and the modular arrangement of short sequences for kinase recognition. We present results from peptides immobilized on two- and three-dimensional surfaces such as hydrogels on 96-well plates and glass slides, and fluorescent Luminex beads. We discuss methods to increase assay sensitivity using chemifluorescent ELISAs, antibody-based recognition, and label-free mass spectrometry. Monitoring the activity of specific kinases in cell lysates presents challenges that can be overcome by manipulating peptide substrates to optimize assay conditions. In particular, signal-to-background ratios were improved by (1) adding long branched hydrophilic linkers between the substrate and the surface, (2) changing the orientation of peptides relative to the surface, and (3) including peptide ligands in cis or in trans to recruit kinases to the surface. By improving the accessibility of immobilized peptide substrates to kinases in solution, the apparent rate of phosphorylation increased and assays were more sensitive to changes in endogenous kinase activities. These strategies can be generalized to improve the reactivity of most peptide substrates used in heterogeneous kinase assays with cell lysates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.