ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2010
Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- Detection ofFrontiers in veterinary science · 2021Article
- Article
- Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A diagnostic assay using immunomagnetic separation was developed to capture Mycobacterium avium subsp. paratuberculosis (MAP) from bovine feces by means of IgY derived from chicken eggs. The antibody was coupled directly onto the surface of MagaCell cellulose/iron oxide beads or indirectly by being mixed with MagaBeads and a rabbit IgG linker against chicken antigen. Optimization parameters for the immunocapture included incubation time, temperature, volume, and type of immunocapture beads. Analytical sensitivity and specificity were determined by extracting DNA from the captured bacteria and amplifying it by polymerase chain reaction (PCR). The 2 bead preparations had the same analytical sensitivity, and the detection level of MAP cells in spiked bovine feces was 2 x 10(4) cells/g. No PCR inhibition was observed with DNA from the organisms captured with use of the MagaCell-IgY beads.
Indexed as
Identifiers
20592839PMC2851719W118044277What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.