ArticlePlant signaling & behavior2010
Straightforward histochemical staining of Fe by the adaptation of an old-school technique: identification of the endodermal vacuole as the site of Fe storage in Arabidopsis embryos.
Article in Plant signaling & behavior, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Effects of Fe and Zn alone and combined treatment on Triticum aestivum L. seed germination.BMC plant biology · 2025Article
- A CYBDOM protein impacts iron homeostasis and primary root growth under phosphate deficiency in Arabidopsis.Nature communications · 2024Article
- Iron Induces Resistance Against the Rice Blast Fungus Magnaporthe oryzae Through Potentiation of Immune Responses.Rice (New York, N.Y.) · 2022Article
- The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plants.Nature communications · 2022Article
- Increasing Provasculature Complexity in the Arabidopsis Embryo May Increase Total Iron Content in Seeds: A Hypothesis.Frontiers in plant science · 2017Article
- Loss of NCB5OR in the cerebellum disturbs iron pathways, potentiates behavioral abnormalities, and exacerbates harmaline-induced tremor in mice.Metabolic brain disease · 2016Article
- Using Perls Staining to Trace the Iron Uptake Pathway in Leaves of a Prunus Rootstock Treated with Iron Foliar Fertilizers.Frontiers in plant science · 2016Article
- Alterations of iron distribution in Arabidopsis tissues infected by Dickeya dadantii.Molecular plant pathology · 2015Article
- The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development.Plant physiology · 2014Article
- Scavenging iron: a novel mechanism of plant immunity activation by microbial siderophores.Plant physiology · 2014Article
- Methodological approaches for using synchrotron X-ray fluorescence (SXRF) imaging as a tool in ionomics: examples from Arabidopsis thaliana.Metallomics : integrated biometal science · 2013Review
- Arabidopsis ferritin 1 (AtFer1) gene regulation by the phosphate starvation response 1 (AtPHR1) transcription factor reveals a direct molecular link between iron and phosphate homeostasis.The Journal of biological chemistry · 2013Article
- Iron deprivation-induced reactive oxygen species generation leads to non-autolytic PCD inEnvironmental and experimental botany · 2013Article
- Atypical iron storage in marine brown algae: a multidisciplinary study of iron transport and storage in Ectocarpus siliculosus.Journal of experimental botany · 2012Article
- Functional characterisation of metal(loid) processes in planta through the integration of synchrotron techniques and plant molecular biology.Analytical and bioanalytical chemistry · 2012Review
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4 authors.
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Abstract
Iron (Fe) is an essential metal ion, required for basic cellular processes such as respiration, photosynthesis and cell division. Therefore, Fe has to be stored and distributed to several organelles to fulfill its roles. The molecular basis of Fe distribution is poorly understood. In this context, elemental imaging approaches are becoming essential for a better understanding of metal homeostasis in plants. Recently, several genes have been involved in Fe storage (VIT1) and remobilization (NRAMP3 and NRAMP4) in the seed of Arabidopsis, mostly with the help of sophisticated imaging techniques. We have adapted an histochemical procedure to detect Fe in plant tissues, based on Perls staining coupled to diaminobenzidine (DAB) intensification. The Perls/DAB technique, quick and inexpensive, was shown to be specific for Fe and highly sensitive. We have applied this procedure to Arabidopsis embryos and shown that Fe is stored in the vacuoles of a specific cell layer surrounding the pro-vascular system, the endodermis. Our results have revealed a new role for the endodermis in Fe storage in the embryo and established the Perls/DAB technique as a powerful tool to detect Fe in plant tissues and cells.
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