ArticleDermato-endocrinology2009
1,25-dihydroxyvitamin D(3) protects human keratinocytes against UV-B-induced damage: In vitro analysis of cell viability/proliferation, DNA-damage and -repair.
Article in Dermato-endocrinology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Illuminating the Connection: Cutaneous Vitamin DNutrients · 2025Review
- Biological Effects of CYP11A1-Derived Vitamin D and Lumisterol Metabolites in the Skin.The Journal of investigative dermatology · 2024Review
- Osteoporosis and dermatoporosis: a review on the role of vitamin D.Frontiers in endocrinology · 2023Review
- Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine.Chemical reviews · 2020Review
- Article
- Oral Photoprotection: Effective Agents and Potential Candidates.Frontiers in medicine · 2018Review
- Pilot study on the bioactivity of vitamin d in the skin after oral supplementation.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Heme Degradation by Heme Oxygenase Protects Mitochondria but Induces ER Stress via Formed Bilirubin.Biomolecules · 2015Article
- Role of Vitamin D Metabolism and Activity on Carcinogenesis.Oncology research · 2014Review
- UVB exposure of farm animals: study on a food-based strategy to bridge the gap between current vitamin D intakes and dietary targets.PloS one · 2013Article
- Unravelling of hidden secrets: The role of vitamin D in skin aging.Dermato-endocrinology · 2012Article
- Age-related disease association of endogenous γ-H2AX foci in mononuclear cells derived from leukapheresis.PloS one · 2012Article
- No evidence for induction of key components of the Notch signaling pathway (Notch-1, Jagged-1) by treatment with UV-B, 1,25(OH)(2)D(3), and/or epigenetic drugs (TSA, 5-Aza) in human keratinocytes in vitro.Dermato-endocrinology · 2012Article
- [The significance of vitamin D metabolism in human skin. An update].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2010Review
- The skin is a fascinating endocrine organ.Dermato-endocrinology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The skin is the only organ that has the capacity to photo-synthesize the biological active vitamin D metabolite 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] from 7-dehydocholesterol (7-DHC), following exposure to ultraviolet (UV)-B irradiation. The aim of the present work was to investigate the capacity of 1,25(OH)(2)D(3) to protect human keratinocytes (HaCaT) and squamous cell carcinoma cell lines (SCL-1) against the hazardous effects of UV-B irradiation. Human keratinocytes (HaCaT) and squamous cell carcinoma cell lines (SCL-1) were pretreated with 1,25(OH)(2)D(3) over 48 hours and then irradiated once with UVB-radiation. We evaluated the results of several assays (colony-forming-unit-culture assay, WST-1-assay and crystal violet assay), comparing viability/proliferation in 1,25(OH)(2)D(3)-pretreated cells with controls that were pretreated with the carrier substance ethanol alone. Additionally, we analyzed the effects of 1,25(OH)(2)D(3) on UV-induced DNA damage in HaCaT-keratinocytes by detection of cyclobutane pyrimidine dimers (CPDs) via dot blot analysis. We prove that 1,25(OH)(2)D(3), in a concentration of 10(-7) M, protects human keratinocytes (HaCaT) as well as squamous cell carcinoma cell lines (SCL-1) against the hazardous effects of UV-B-radiation (100 J/cm(2)-1,000 J/cm(2)) in vitro. Moreover, we demonstrate that the number of CPDs induced in HaCaT-keratinocytes after irradiation with UV-B (100 J/cm(2)-1,000 J/cm(2)) was decreased after pretreatment with 1,25(OH)(2)D(3), as compared to carrier-treated controls. Analysis of the time course revealed that the elimination of UV-B-induced DNA-damage in HaCaT-keratinocytes occurs quicker when cells are pretreated with 1,25(OH)(2)D(3) (as compared to controls). To put it in a nutshell, our data support the hypothesis that 1,25(OH)(2)D(3) protects cultured human keratinocytes against the hazardous effects of UV-B radiation.
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Registered trials
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