Evidence map›Paper›PMID 20592146›Full record

ArticleJournal of clinical microbiology2010

Multiplex PCR for differential identification of broad tapeworms (Cestoda: Diphyllobothrium) infecting humans.

Barbara Wicht, Tetsuya Yanagida, Tomás Scholz, Akira Ito, Juan A Jiménez, Jan Brabec

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 92 citations in OpenAlex.

  1. Review
  2. Article
  3. A Case of Dibothriocephalosis (Pathogens (Basel, Switzerland) · 2025
    Article
  4. Article
  5. Occurrence and the first molecular genotyping ofCurrent research in parasitology & vector-borne diseases · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. The Brief Case: the Boy Who Cried Worm.Journal of clinical microbiology · 2023
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Global analysis of cytochromeCurrent research in parasitology & vector-borne diseases · 2021
    Article
  16. Article
  17. Occurrence and Spatial Distribution ofInternational journal of environmental research and public health · 2020
    Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 4 countries.

Barbara WichtIstituto Cantonale di Microbiologia, Via Mirasole 22a, 6500 Bellinzona, Switzerland. barbarawicht@yahoo.it
Tetsuya Yanagida
Tomás Scholz
Akira Ito
Juan A Jiménez
Jan Brabec
Asahikawa Medical College Hospital · JPInstitute of Parasitology · CZCzech Academy of Sciences, Biology Centre · CZUniversidad Peruana Cayetano Heredia · PE

Funding

Training in Parasitic Diseases and Central Nervous System Infections in Peru.D43TW001140 · FIC · UNIVERSIDAD PERUANA CAYETANO HEREDIA · PI GARCIA, HECTOR HUGO, GILMAN, ROBERT H · 1999 to 2025
$5.7M
FIC NIH HHS D43 TW001140FIC NIH HHS TW001140
6 · The paper itself

Abstract

The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing, although highly precise, are too complicated, long, and expensive to be employed in medical laboratories for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA.

Indexed as

AnimalsDiphyllobothriasisDiphyllobothriumDNA, HelminthElectron Transport Complex IVHelminth ProteinsHumansMolecular Sequence DataParasitologyPolymerase Chain ReactionSequence Analysis, DNADNA, HelminthElectron Transport Complex IVHelminth Proteins

Identifiers

PMID20592146
PMCPMC2937707
OpenAlexW2128229076

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.