ArticleThe Journal of biological chemistry2010
para-Aminobenzoic acid is a precursor in coenzyme Q6 biosynthesis in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 109 citations in OpenAlex.
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- Understanding coenzyme Q.Physiological reviews · 2024Review
- Yeast of Eden: microbial resistance to glyphosate from a yeast perspective.Current genetics · 2023Review
- Coenzyme Q inMarine drugs · 2023Article
- Identification of novel coenzyme QThe Journal of biological chemistry · 2023Article
- Metabolomic Analysis Reveals the Effect of Insecticide Chlorpyrifos on Rice Plant Metabolism.Metabolites · 2022Article
- Predicting and Understanding the Pathology of Single Nucleotide Variants in HumanAntioxidants (Basel, Switzerland) · 2022Article
- Coenzyme Q10 in the eye isomerizes by sunlight irradiation.Scientific reports · 2022Article
- Recent advances in the metabolic pathways and microbial production of coenzyme Q.World journal of microbiology & biotechnology · 2022Review
- Genetic evidence for the requirements of antroquinonol biosynthesis by Antrodia camphorata during liquid-state fermentation.Journal of industrial microbiology & biotechnology · 2022Article
- Dual transcriptome based reconstruction of Salmonella-human integrated metabolic network to screen potential drug targets.PloS one · 2022Article
- Coenzyme Q Biosynthesis: An Update on the Origins of the Benzenoid Ring and Discovery of New Ring Precursors.Metabolites · 2021Review
- Genome Evolutionary Dynamics Meets Functional Genomics: A Case Story on the Identification of SLC25A44.International journal of molecular sciences · 2021Article
- Synthesis and Antibacterial Activity of New Azole, Diazole and Triazole Derivatives Based onMolecules (Basel, Switzerland) · 2021Article
- To a Question on the Mechanism of the Antimicrobial Action of Ortho-Benzoic Sulfimide.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Metabolic engineering of Saccharomyces cerevisiae for high-level production of gastrodin from glucose.Microbial cell factories · 2020Article
- Genes and lipids that impact uptake and assimilation of exogenous coenzyme Q in Saccharomyces cerevisiae.Free radical biology & medicine · 2020Article
- Article
3 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
Abstract
Coenzyme Q (ubiquinone or Q) is a crucial mitochondrial lipid required for respiratory electron transport in eukaryotes. 4-Hydroxybenozoate (4HB) is an aromatic ring precursor that forms the benzoquinone ring of Q and is used extensively to examine Q biosynthesis. However, the direct precursor compounds and enzymatic steps for synthesis of 4HB in yeast are unknown. Here we show that para-aminobenzoic acid (pABA), a well known precursor of folate, also functions as a precursor for Q biosynthesis. A hexaprenylated form of pABA (prenyl-pABA) is normally present in wild-type yeast crude lipid extracts but is absent in yeast abz1 mutants starved for pABA. A stable (13)C(6)-isotope of pABA (p- amino[aromatic-(13)C(6)]benzoic acid ([(13)C(6)]pABA)), is prenylated in either wild-type or abz1 mutant yeast to form prenyl-[(13)C(6)]pABA. We demonstrate by HPLC and mass spectrometry that yeast incubated with either [(13)C(6)]pABA or [(13)C(6)]4HB generate both (13)C(6)-demethoxy-Q (DMQ), a late stage Q biosynthetic intermediate, as well as the final product (13)C(6)-coenzyme Q. Pulse-labeling analyses show that formation of prenyl-pABA occurs within minutes and precedes the synthesis of Q. Yeast utilizing pABA as a ring precursor produce another nitrogen containing intermediate, 4-imino-DMQ(6). This intermediate is produced in small quantities in wild-type yeast cultured in standard media and in abz1 mutants supplemented with pABA. We suggest a mechanism where Schiff base-mediated deimination forms DMQ(6) quinone, thereby eliminating the nitrogen contributed by pABA. This scheme results in the convergence of the 4HB and pABA pathways in eukaryotic Q biosynthesis and has implications regarding the action of pABA-based antifolates.
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