ArticleThe Journal of biological chemistry2010
p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- NADPH oxidases: redox regulation of cell homeostasis and disease.Physiological reviews · 2025Review
- New NADPH Oxidase 2 Inhibitors Display Potent Activity against Oxidative Stress by Targeting p22Antioxidants (Basel, Switzerland) · 2023Article
- Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Characterization of the Intramolecular Interactions and Regulatory Mechanisms of the Scaffold Protein Tks4.International journal of molecular sciences · 2021Article
- NADPH Oxidases (NOX): An Overview from Discovery, Molecular Mechanisms to Physiology and Pathology.Antioxidants (Basel, Switzerland) · 2021Review
- Conformational maps of human 20S proteasomes reveal PA28- and immuno-dependent inter-ring crosstalks.Nature communications · 2020Article
- Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier.Biophysical journal · 2020Article
- Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils.The Journal of experimental medicine · 2019Article
- Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.The Journal of biological chemistry · 2019Article
- Suppressed ubiquitination of Nrf2 by p47Free radical biology & medicine · 2017Article
- Down-regulation of NOX2 activity in phagocytes mediated by ATM-kinase dependent phosphorylation.Free radical biology & medicine · 2017Article
- Neutrophils to the ROScue: Mechanisms of NADPH Oxidase Activation and Bacterial Resistance.Frontiers in cellular and infection microbiology · 2017Review
- New insights into the regulation of neutrophil NADPH oxidase activity in the phagosome: a focus on the role of lipid and Ca(2+) signaling.Antioxidants & redox signaling · 2013Review
- Strategies for identifying synthetic peptides to act as inhibitors of NADPH oxidases, or "all that you did and did not want to know about Nox inhibitory peptides".Cellular and molecular life sciences : CMLS · 2012Review
- Structural basis for the 14-3-3 protein-dependent inhibition of the regulator of G protein signaling 3 (RGS3) function.The Journal of biological chemistry · 2011Article
- Mass spectrometry: come of age for structural and dynamical biology.Current opinion in structural biology · 2011Review
- Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets.Nature reviews. Drug discovery · 2011Review
- Inflammation and reactive oxygen species in cardiovascular disease.World journal of cardiology · 2010Article
- Regulation of NADPH oxidase activity in phagocytes: relationship between FAD/NADPH binding and oxidase complex assembly.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The p47(phox) cytosolic factor from neutrophilic NADPH oxidase has always been resistant to crystallogenesis trials due to its modular organization leading to relative flexibility. Hydrogen/deuterium exchange coupled to mass spectrometry was used to obtain structural information on the conformational mechanism that underlies p47(phox) activation. We confirmed a relative opening of the protein with exposure of the SH3 Src loops that are known to bind p22(phox) upon activation. A new surface was shown to be unmasked after activation, representing a potential autoinhibitory surface that may block the interaction of the PX domain with the membrane in the resting state. Within this surface, we identified 2 residues involved in the interaction with the PX domain. The double mutant R162A/D166A showed a higher affinity for specific phospholipids but none for the C-terminal part of p22(phox), reflecting an intermediate conformation between the autoinhibited and activated forms.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.