ArticleNeurogastroenterology and motility2010
Differential effects of thin and thick filament disruption on zebrafish smooth muscle regulatory proteins.
Article in Neurogastroenterology and motility, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- A compendium of developmental gene expression in Lake Malawi cichlid fishes.BMC developmental biology · 2017Article
- The enteric nervous system promotes intestinal health by constraining microbiota composition.PLoS biology · 2017Article
- Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility and vascular function in zebrafish.Disease models & mechanisms · 2016Article
- Ultra-structural identification of interstitial cells of Cajal in the zebrafish Danio rerio.Cell and tissue research · 2012Article
- Smooth muscle caldesmon modulates peristalsis in the wild type and non-innervated zebrafish intestine.Neurogastroenterology and motility · 2012Article
- Article
- Development of the zebrafish enteric nervous system.Methods in cell biology · 2011Review
- Characterization of zebrafish intestinal smooth muscle development using a novel sm22α-b promoter.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe smooth muscle actin binding proteins Caldesmon and Tropomyosin (Tm) promote thin filament assembly by stabilizing actin polymerization, however, whether filament assembly affects either the stability or activation of these and other smooth muscle regulatory proteins is not known.
methodsMeasurement of smooth muscle regulatory protein levels in wild type zebrafish larvae following antisense knockdown of smooth muscle actin (Acta2) and myosin heavy chain (Myh11) proteins, and in colourless mutants that lack enteric nerves. Comparison of intestinal peristalsis in wild type and colourless larvae. KEY
resultsKnockdown of Acta2 led to reduced levels of phospho-Caldesmon and Tm. Total Caldesmon and phospho-myosin light chain (p-Mlc) levels were unaffected. Knockdown of Myh11 had no effect on the levels of either of these proteins. Phospho-Caldesmon and p-Mlc levels were markedly reduced in colourless mutants that have intestinal motility comparable with wild type larvae. CONCLUSIONS & INFERENCES: These in vivo findings provide new information regarding the activation and stability of smooth muscle regulatory proteins in zebrafish larvae and their role in intestinal peristalsis in this model organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.