ArticleBiochemistry2010
Attractant and repellent signaling conformers of sensory rhodopsin-transducer complexes.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Probing Channelrhodopsin Electrical Activity in Algal Cell Populations.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.Annual review of biochemistry · 2017Review
- Photochemical reaction cycle transitions during anion channelrhodopsin gating.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gating mechanisms of a natural anion channelrhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cation-Specific Conformations in a Dual-Function Ion-Pumping Microbial Rhodopsin.Biochemistry · 2015Article
- His166 is the Schiff base proton acceptor in attractant phototaxis receptor sensory rhodopsin I.Biochemistry · 2014Article
- Mechanism divergence in microbial rhodopsins.Biochimica et biophysica acta · 2014Review
- Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.Chemical reviews · 2014Review
- Chimeric proton-pumping rhodopsins containing the cytoplasmic loop of bovine rhodopsin.PloS one · 2014Article
- Intramolecular proton transfer in channelrhodopsins.Biophysical journal · 2013Article
- Schiff base switch II precedes the retinal thermal isomerization in the photocycle of bacteriorhodopsin.PloS one · 2013Article
- Opposite displacement of helix F in attractant and repellent signaling by sensory rhodopsin-Htr complexes.The Journal of biological chemistry · 2011Article
- Sensory rhodopsin-I as a bidirectional switch: opposite conformational changes from the same photoisomerization.Biophysical journal · 2011Article
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Authors and funding
4 authors.
Funding
Abstract
Attractant and repellent signaling conformers of the dual-signaling phototaxis receptor sensory rhodopsin I and its transducer subunit (SRI-HtrI) have recently been distinguished experimentally by the opposite connection of their retinylidene protonated Schiff bases to the outwardly located periplasmic side and inwardly located cytoplasmic side. Here we show that the pK(a) of the outwardly located Asp76 counterion in the outwardly connected conformer is lowered by approximately 1.5 units from that of the inwardly connected conformer. The pK(a) difference enables quantitative determination of the relative amounts of the two conformers in wild-type cells and behavioral mutants prior to photoexcitation, comparison of their absorption spectra, and determination of their relative signaling efficiency. We have shown that the one-photon excitation of the SRI-HtrI attractant conformer causes a Schiff base connectivity switch from inwardly connected to outwardly connected states in the attractant signaling photoreaction. Conversely, a second near-UV photon drives the complex back to the inwardly connected conformer in the repellent signaling photoreaction. The results suggest a model of the color-discriminating dual-signaling mechanism in which phototaxis responses (his-kinase modulation) result from the photointerconversion of the two oppositely connected SRI-HtrI conformers by one-photon and two-photon activation. Furthermore, we find that the related repellent phototaxis SRII-HtrII receptor complex has an outwardly connected retinylidene Schiff base like the repellent signaling forms of the SRI-HtrI complex, indicating the general applicability of macro conformational changes, which can be detected by the connectivity switch, to phototaxis signaling by sensory rhodopsin-transducer complexes.
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