ArticleMolecular biology of the cell2010
The Rsr1/Bud1 GTPase interacts with itself and the Cdc42 GTPase during bud-site selection and polarity establishment in budding yeast.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP.EMBO reports · 2026Article
- Rax1/2 promote memory of growth by early Ras1 activation to maintain polarity across generations.bioRxiv : the preprint server for biology · 2025Article
- Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery.The Journal of cell biology · 2022Article
- Review
- Overexpression of theJournal of fungi (Basel, Switzerland) · 2020Article
- Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.Cells · 2020Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine.The Journal of cell biology · 2019Article
- Temporal regulation of cell polarity via the interaction of the Ras GTPase Rsr1 and the scaffold protein Bem1.Molecular biology of the cell · 2019Article
- SH3-class Ras guanine nucleotide exchange factors are essential for Aspergillus fumigatus invasive growth.Cellular microbiology · 2019Article
- The shared role of the Rsr1 GTPase and Gic1/Gic2 in Cdc42 polarization.Molecular biology of the cell · 2018Article
- Fine-tuning the orientation of the polarity axis by Rga1, a Cdc42 GTPase-activating protein.Molecular biology of the cell · 2017Article
- Computational and biochemical characterization of two partially overlapping interfaces and multiple weak-affinity K-Ras dimers.Scientific reports · 2017Article
- Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42.Scientific reports · 2016Article
- Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies.Journal of molecular biology · 2015Review
- Article
- Sequential logic of polarity determination during the haploid-to-diploid transition in Saccharomyces cerevisiae.Eukaryotic cell · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Cell polarization occurs along a single axis that is generally determined in response to spatial cues. In budding yeast, the Rsr1 GTPase and its regulators direct the establishment of cell polarity at the proper cortical location in response to cell type-specific cues. Here we use a combination of in vivo and in vitro approaches to understand how Rsr1 polarization is established. We find that Rsr1 associates with itself in a spatially and temporally controlled manner. The homotypic interaction and localization of Rsr1 to the mother-bud neck and to the subsequent division site are dependent on its GDP-GTP exchange factor Bud5. Analyses of rsr1 mutants suggest that Bud5 recruits Rsr1 to these sites and promotes the homodimer formation. Rsr1 also exhibits heterotypic interaction with the Cdc42 GTPase in vivo. We show that the polybasic region of Rsr1 is necessary for the efficient homotypic and heterotypic interactions, selection of a proper growth site, and polarity establishment. Our findings thus suggest that dimerization of GTPases may be an efficient mechanism to set up cellular asymmetry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.