ArticleThe Plant cell2010
RNA-seq analysis of sulfur-deprived Chlamydomonas cells reveals aspects of acclimation critical for cell survival.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 134 papers.
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Who cites it
134 citing papers in PubMed.
- Identification and characterization of low-sulfur-inducible genes and pathways in roots of upland and lowland rice grown under hydroponic and soil conditions.Journal of experimental botany · 2026Article
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Client distribution between Chlamydomonas FDX1 and FDX2 in carbon, nitrogen and sulfur assimilation.bioRxiv : the preprint server for biology · 2026Article
- Age-Specific Physiological Adjustments ofPlants (Basel, Switzerland) · 2025Article
- Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems.Plants (Basel, Switzerland) · 2024Review
- Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive.The Plant cell · 2023Article
- Small RNAs >26 nt in length associate with AGO1 and are upregulated by nutrient deprivation in the alga Chlamydomonas.The Plant cell · 2023Article
- Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell.Communications biology · 2023Article
- Probe-based bacterial single-cell RNA sequencing predicts toxin regulation.Nature microbiology · 2023Article
- DNA Methylation in Algae and Its Impact on Abiotic Stress Responses.Plants (Basel, Switzerland) · 2023Review
- Overexpression of PSR1 inFrontiers in plant science · 2023Article
- The elements of life: A biocentric tour of the periodic table.Advances in microbial physiology · 2023Article
- The landscape of Chlamydomonas histone H3 lysine 4 methylation reveals both constant features and dynamic changes during the diurnal cycle.The Plant journal : for cell and molecular biology · 2022Article
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Comparative Phenotyping of Two Commonly UsedPlants (Basel, Switzerland) · 2022Article
- A life-history trade-off gene with antagonistic pleiotropic effects on reproduction and survival in limiting environments.Proceedings. Biological sciences · 2022Article
- Role of Sulfate Transporters in Chromium Tolerance inPlants (Basel, Switzerland) · 2022Article
- MicroRNA Expression Profile Analysis ofBioengineering (Basel, Switzerland) · 2021Article
- Photoautotrophs-Bacteria Co-Cultures: Advances, Challenges and Applications.Materials (Basel, Switzerland) · 2021Review
- Co-expression networks in Chlamydomonas reveal significant rhythmicity in batch cultures and empower gene function discovery.The Plant cell · 2021Article
74 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including the cell wall and complexes associated with the photosynthetic apparatus. Moreover, the data suggest that sulfur-deprived cells accumulate proteins with fewer sulfur-containing amino acids. Most of the sulfur deprivation responses are controlled by the SNRK2.1 protein kinase. The snrk2.1 mutant exhibits a set of unique responses during both sulfur-replete and sulfur-depleted conditions that are not observed in wild-type cells; the inability of this mutant to acclimate to S deprivation probably leads to elevated levels of singlet oxygen and severe oxidative stress, which ultimately causes cell death. The transcriptome results for wild-type and mutant cells strongly suggest the occurrence of massive changes in cellular physiology and metabolism as cells become depleted for sulfur and reveal aspects of acclimation that are likely critical for cell survival.
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