ArticleJournal of cell science2010
Co-regulation of nuclear respiratory factor-1 by NFkappaB and CREB links LPS-induced inflammation to mitochondrial biogenesis.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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Who cites it
49 citing papers in PubMed, 105 citations in OpenAlex.
- CREB regulates Foxp3Frontiers in immunology · 2025Article
- Pterostilbene improves neurological dysfunction and neuroinflammation after ischaemic stroke via HDAC3/Nrf1-mediated microglial activation.Cellular & molecular biology letters · 2024Article
- Endometrial organoids: a reservoir of functional mitochondria for uterine repair.Theranostics · 2024Article
- Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis.Biology direct · 2023Article
- NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity.The EMBO journal · 2023Article
- Metabolic profile of mesenchymal stromal cells and macrophages in the presence of polyethylene particles in a 3D model.Stem cell research & therapy · 2023Article
- Nuclear respiratory factor 1 promotes the progression of EBV-associated gastric cancer and maintains EBV latent infection.Virus genes · 2023Article
- Molecular Mechanisms Underlying TNFα-Induced Mitochondrial Biogenesis in Human Airway Smooth Muscle.International journal of molecular sciences · 2023Article
- Expression of Concern: NF-κB and CREB Are Required for Angiotensin II Type 1 Receptor Upregulation in Neurons.PloS one · 2023Article
- Detection of Neanderthal Adaptively Introgressed Genetic Variants That Modulate Reporter Gene Expression in Human Immune Cells.Molecular biology and evolution · 2022Article
- Testosterone attenuates hypoxia-induced hypertension by affecting NRF1-mediated transcriptional regulation of ET-1 and ACE.Hypertension research : official journal of the Japanese Society of Hypertension · 2021Article
- Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation.Molecular brain · 2021Article
- Testosterone attenuates pulmonary epithelial inflammation in male rats of COPD model through preventing NRF1-derived NF-κB signaling.Journal of molecular cell biology · 2021Article
- Exogenous Alpha-Synuclein Evoked Parkin Downregulation Promotes Mitochondrial Dysfunction in Neuronal Cells. Implications for Parkinson's Disease Pathology.Frontiers in aging neuroscience · 2021Article
- miR‑146a improves hepatic lipid and glucose metabolism by targeting MED1.International journal of molecular medicine · 2020Article
- Endothelial Stanniocalcin 1 Maintains Mitochondrial Bioenergetics and Prevents Oxidant-Induced Lung Injury via Toll-Like Receptor 4.Antioxidants & redox signaling · 2019Article
- Effects of nuclear respiratory factor‑1 on apoptosis and mitochondrial dysfunction induced by cobalt chloride in H9C2 cells.Molecular medicine reports · 2019Article
- High-throughput functional analysis of lncRNA core promoters elucidates rules governing tissue specificity.Genome research · 2019Article
- Frontline Science: Monocytes sequentially rewire metabolism and bioenergetics during an acute inflammatory response.Journal of leukocyte biology · 2019Article
- Cerebrolysin Ameliorates Focal Cerebral Ischemia Injury Through Neuroinflammatory InhibitionFrontiers in pharmacology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The nuclear respiratory factor-1 (NRF1) gene is activated by lipopolysaccharide (LPS), which might reflect TLR4-mediated mitigation of cellular inflammatory damage via initiation of mitochondrial biogenesis. To test this hypothesis, we examined NRF1 promoter regulation by NFκB, and identified interspecies-conserved κB-responsive promoter and intronic elements in the NRF1 locus. In mice, activation of Nrf1 and its downstream target, Tfam, by Escherichia coli was contingent on NFκB, and in LPS-treated hepatocytes, NFκB served as an NRF1 enhancer element in conjunction with NFκB promoter binding. Unexpectedly, optimal NRF1 promoter activity after LPS also required binding by the energy-state-dependent transcription factor CREB. EMSA and ChIP assays confirmed p65 and CREB binding to the NRF1 promoter and p65 binding to intron 1. Functionality for both transcription factors was validated by gene-knockdown studies. LPS regulation of NRF1 led to mtDNA-encoded gene expression and expansion of mtDNA copy number. In cells expressing plasmid constructs containing the NRF-1 promoter and GFP, LPS-dependent reporter activity was abolished by cis-acting κB-element mutations, and nuclear accumulation of NFκB and CREB demonstrated dependence on mitochondrial H(2)O(2). These findings indicate that TLR4-dependent NFκB and CREB activation co-regulate the NRF1 promoter with NFκB intronic enhancement and redox-regulated nuclear translocation, leading to downstream target-gene expression, and identify NRF-1 as an early-phase component of the host antibacterial defenses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.