ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2010
Identification of markers for imminent death in mice used in longevity and aging research.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Can harmonisation of outcomes bridge the translation gap for pre-clinical research? A systematic review of outcomes measured in mouse models of type 2 diabetes.Journal of translational medicine · 2020Pooled it
- Frailty assessment in naturally aged mouse models: classification, limitations, and future directions.GeroScience · 2026Review
- Chronic exposure to aerosolized Arizona test dust reduces visual acuity in mice.Toxicology reports · 2026Article
- Genetic regulation of fasting-induced longevity effects.bioRxiv : the preprint server for biology · 2025Article
- Mitochondrial dysfunction enhances influenza pathogenesis by up-regulating de novo sialic acid biosynthesis.Science advances · 2025Article
- Longitudinal fragility phenotyping contributes to the prediction of lifespan and age-associated morbidity in C57BL/6 and Diversity Outbred mice.GeroScience · 2024Article
- Evidence-Based Severity Assessment of Animal Models for Pancreatic Cancer.Biomedicines · 2024Article
- Longitudinal Fragility Phenotyping Predicts Lifespan and Age-Associated Morbidity in C57BL/6 and Diversity Outbred Mice.bioRxiv : the preprint server for biology · 2024Article
- Pathological beige remodeling induced by cancer cachexia depends on the disease severity and involves mainly the trans-differentiation of mature white adipocytes.bioRxiv : the preprint server for biology · 2023Article
- Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats.Scientific reports · 2023Article
- Chronic exposure to dim light at night disrupts cell-mediated immune response and decreases longevity in aged female mice.Chronobiology international · 2022Article
- High Housing Density-Induced Chronic Stress Diminishes Ovarian Reserve via Granulosa Cell Apoptosis by Angiotensin II Overexpression in Mice.International journal of molecular sciences · 2022Article
- Enhanced BRAF engagement by NRAS mutants capable of promoting melanoma initiation.Nature communications · 2022Article
- The Influence of Heterochronic Non-Myeloablative Bone Marrow Transplantation on the Immune System, Frailty, General Health, and Longevity of Aged Murine Recipients.Biomolecules · 2022Article
- Lifespan extension with preservation of hippocampal function in aged system xMolecular psychiatry · 2022Article
- Hydro-ethanol extract of Holarrhena floribunda stem bark exhibits anti-anaphylactic and anti-oedematogenic effects in murine models of acute inflammation.BMC complementary medicine and therapies · 2022Article
- Prophylactic or therapeutic administration of Holarrhena floribunda hydro ethanol extract suppresses complete Freund's adjuvant-induced arthritis in Sprague-Dawley rats.Journal of inflammation (London, England) · 2022Article
- Safety study of allogeneic mesenchymal stem cell therapy in animal model.Regenerative therapy · 2022Article
- Obesity protects against sepsis-induced and norepinephrine-induced white adipose tissue browning.American journal of physiology. Endocrinology and metabolism · 2021Article
- Drastic hypothermia after intraperitoneal injection of okadaic acid, a diarrhetic shellfish poisoning toxin, in mice.Experimental animals · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The goal of this study was to identify objective criteria that would reliably predict imminent death in aged mice. Male and female ICR mice (age, 8 mo) were subcutaneously implanted with an identification chip for remote measurement of body temperature. Mice then were weighed and monitored regularly until spontaneous death occurred or until euthanasia was administered for humane reasons. Clinical signs that signaled implementation of euthanasia included inability to walk, lack of response to manipulation, large or ulcerated tumors, seizures, and palpable hypothermia. In mice that died spontaneously, gradual weight loss was the most frequent and earliest sign of imminent death. Hypothermia developed during the 2 wk prior to death. Slow or labored breathing were observed in about half of the mice before death. A composite score of temperature x weight can be used to provide an objective benchmark to signal increased observation or euthanasia of individual mice. Such assessment may allow the collection of terminal tissue samples without markedly altering longevity data, although application of this criterion may not be appropriate for all studies of longevity. Timely euthanasia of mice based on validated markers of imminent death can allow implementation of endpoints that alleviate terminal distress in aged mice, may not significantly affect longevity data, and can permit timely collection of biologic samples.
Indexed as
Identifiers
20587157PMC2877298What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.