ArticleCell transplantation2010
Isolation and propagation of a human CD133(-) colon tumor-derived cell line with tumorigenic and angiogenic properties.
Article in Cell transplantation, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Beyond a cancer stem cell marker: molecular state dependent functions of CD133 and state targeted therapy.Biomarker research · 2026Review
- Functional Roles of CD133: More than Stemness Associated Factor Regulated by the Microenvironment.Stem cell reviews and reports · 2024Review
- Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.PloS one · 2017Article
- Animal models of colorectal peritoneal metastasis.Pleura and peritoneum · 2016Review
- Major Protein of Carcinoembryonic Antigen Gene Family - CD66c, A Novel Marker in Colon Carcinoma.Journal of clinical and diagnostic research : JCDR · 2016Article
- Cancer stem cells in human digestive tract malignancies.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Review
- B7H1 Expression and Epithelial-To-Mesenchymal Transition Phenotypes on Colorectal Cancer Stem-Like Cells.PloS one · 2015Article
- Prognostic significance of CD44 variant 2 upregulation in colorectal cancer.British journal of cancer · 2014Article
- In vivo 5FU-exposed human medullary thyroid carcinoma cells contain a chemoresistant CD133+ tumor-initiating cell subset.Thyroid : official journal of the American Thyroid Association · 2014Article
- Article
- Cancer stem cell gene profile as predictor of relapse in high risk stage II and stage III, radically resected colon cancer patients.PloS one · 2013Article
- IL-1β promotes stemness and invasiveness of colon cancer cells through Zeb1 activation.Molecular cancer · 2012Article
- Rapid re-expression of CD133 protein in colorectal cancer cell lines in vitro and in vivo.Laboratory investigation; a journal of technical methods and pathology · 2012Article
- Lessons from common markers of tumor-initiating cells in solid cancers.Cellular and molecular life sciences : CMLS · 2011Review
- Association of stem-like cells in gender-specific chemoprevention against intestinal neoplasia in MIN mouse.Oncology reports · 2011Article
- Cancer Stem Cells and Pediatric Solid Tumors.Cancers · 2011Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 2 countries.
Funding
Abstract
It has been proposed in human colorectal cancers (CRC) a minority subset of cancer cells within tumors able to initiate tumor growth, defined as cancer stem cells (CSC). Solid human primary colonic and its ovarian metastatic cancer tissues were collected from fresh surgical samples and subsequent xenografts were established in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. The resulting tumors were disaggregated into single-cell suspensions and a CD133(-) cell line (NANK) was newly established and analyzed by flow cytometry. Surface markers of progenitor cells were immunophenotypically analyzed, and expression of stem cell and cancer-related genes was characterized. Secreted angiogenesis-associated molecules were investigated by proteomic array technology. Finally, different numbers of NANK were implanted and their tumor-initiating properties were investigated in NOD/SCID mice. Intraperitoneal injection of NANK in NOD/SCID mice induced tumors with developing progressive peritoneal dissemination and ascites. NANK cells maintained a differentiated phenotype and reproduced the full morphologic and phenotypic heterogeneity of their parental lesions. Noticeably, NANK lacked the expression of conventional CSC markers CD133 and CD44, self-renewal genes Oct-4 and Nanog, but showed the expression of an important gastrointestinal development marker CDX-2 and BMI-1 that is essential in regulating the proliferative activity of normal and leukemic stem cells. In addition, NANK secreted high amounts of important angiogenic cytokines. These results provide a novel and extensive model in human CSC for studying the generation and maintenance of phenotypic heterogeneity in CRC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.