Evidence map›Paper›PMID 20587066›Full record

ArticleVirology journal2010

Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses.

Aline Welzel Gramkow, Simone Perecmanis, Raul Lima Barbosa Sousa, Eliane Ferreira Noronha, Carlos Roberto Felix, Tatsuya Nagata, Bergmann Morais Ribeiro

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Aline Welzel GramkowCell Biology Department, University of Brasília, Brasília, DF, CEP 70910-970, Brazil.
Simone Perecmanis
Raul Lima Barbosa Sousa
Eliane Ferreira Noronha
Carlos Roberto Felix
Tatsuya Nagata
Bergmann Morais Ribeiro
Universidade de Brasília · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBaculovirus comprise the largest group of insect viruses most studied worldwide, mainly because they efficiently kill agricultural insect pests. In this study, two recombinant baculoviruses containing the ScathL gene from Sarcophaga peregrina (vSynScathL), and the Keratinase gene from the fungus Aspergillus fumigatus (vSynKerat), were constructed, and their insecticidal properties analysed against Spodoptera frugiperda larvae.

resultsBioassays of third-instar and neonate S. frugiperda larvae with vSynScathL and vSynKerat showed a decrease in the time needed to kill the infected insects when compared to the wild type virus. We have also shown that both recombinants were able to increase phenoloxidase activity in the hemolymph of S. frugiperda larvae. The expression of proteases in infected larvae resulted in destruction of internal tissues late in infection, which could be the reason for the increased viral speed of kill.

conclusionsBaculoviruses and their recombinant forms constitute viable alternatives to chemical insecticides. Recombinant baculoviruses containing protease genes can be added to the list of engineered baculoviruses with great potential to be used in integrated pest management programs.

Indexed as

Genetic EngineeringAnimalsAspergillus fumigatusCathepsin LCell LineDipteraFungal ProteinsInsecticidesInsect ProteinsLarvaNucleopolyhedrovirusesPeptide HydrolasesPest Control, BiologicalSpodopteraCathepsin LFungal ProteinsInsecticidesInsect ProteinskeratinasePeptide Hydrolases

Identifiers

PMID20587066
PMCPMC2903562
OpenAlexW2100499596

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.