ArticleJournal of the American Chemical Society2010
Hairpin DNA-functionalized gold colloids for the imaging of mRNA in live cells.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 172 citations in OpenAlex.
- Spherical Nucleic Acids: Turning Synthetic Advances and Fundamental Discovery into Translational Breakthroughs in Chemistry, Materials Development, Biology, and Medicine.Accounts of chemical research · 2026Article
- Assessment of Hairpin-Functionalization of Gold Nanoparticles toward a Facile Detection of Zika Virus and Lineage Identification.ACS omega · 2025Article
- Covalent Organic Framework-Based Theranostic Platforms for Restricting H1N1 Influenza Virus Infection.International journal of nanomedicine · 2024Article
- Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium.Molecules (Basel, Switzerland) · 2023Article
- Review
- Facile Preparation of a Hairpin DNA-Gold Nanoparticle Monoconjugate with a Single-Dye Molecule and Lactobionic Acid as Targeting Ligand.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Protein Spherical Nucleic Acids for Live-Cell Chemical Analysis.Journal of the American Chemical Society · 2020Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Targeted Imaging of VCAM-1 mRNA in a Mouse Model of Laser-Induced Choroidal Neovascularization Using Antisense Hairpin-DNA-Functionalized Gold-Nanoparticles.Molecular pharmaceutics · 2018Article
- Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids.The Analyst · 2018Article
- Ratiometric optical nanoprobes enable accurate molecular detection and imaging.Chemical Society reviews · 2018Review
- Real-time imaging of VCAM-1 mRNA in TNF-α activated retinal microvascular endothelial cells using antisense hairpin-DNA functionalized gold nanoparticles.Nanomedicine : nanotechnology, biology, and medicine · 2018Article
- Study on the Fluorescent Activity of N²-Indolyl-1,2,3-triazole.Molecules (Basel, Switzerland) · 2017Article
- Article
- Gold nanorods inhibit respiratory syncytial virus by stimulating the innate immune response.Nanomedicine : nanotechnology, biology, and medicine · 2016Article
- Rationally designed molecular beacons for bioanalytical and biomedical applications.Chemical Society reviews · 2015Review
- Gold nanoparticle-based beacon to detect STAT5b mRNA expression in living cells: a case optimized by bioinformatics screen.International journal of nanomedicine · 2015Article
- DNA dendrimer: an efficient nanocarrier of functional nucleic acids for intracellular molecular sensing.ACS nano · 2014Article
- Real-time light scattering tracking of gold nanoparticles- bioconjugated respiratory syncytial virus infecting HEp-2 cells.Scientific reports · 2014Article
- A targeted, self-delivered, and photocontrolled molecular beacon for mRNA detection in living cells.Journal of the American Chemical Society · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A strategy is presented for the live cell imaging of messenger RNA using hairpin DNA-functionalized gold nanoparticles (hAuNP). hAuNP improve upon technologies for studying RNA trafficking by their efficient internalization within live cells without transfection reagents, improved resistance to DNase degradation, low cytotoxicity, and the incorporation of hairpin DNA molecular beacons to confer high specificity and sensitivity to the target mRNA sequence. Furthermore, the targeted nanoparticle-beacon construct, once bound to the target mRNA sequence, remains hybridized to the target, enabling spatial and temporal studies of RNA trafficking and downstream analysis. Targeted hAuNP exhibited high specificity for glyceraldehyde 3-phosphate dehydrogenase (GADPH) mRNA in live normal HEp-2 cells and respiratory syncytial virus (RSV) mRNA in live RSV-infected HEp-2 cells with high target to background ratios. Multiplexed fluorescence imaging of distinct mRNAs in live cells and simultaneous imaging of mRNAs with immunofluorescently stained protein targets in fixed cells was enabled by appropriate selection of molecular beacon fluorophores. Pharmacologic analysis suggested that hAuNP were internalized within cells via membrane-nanoparticle interactions. hAuNP are a promising approach for the real-time analysis of mRNA transport and processing in live cells for elucidation of biological processes and disease pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.