ArticlePloS one2010
Connective tissue growth factor in regulation of RhoA mediated cytoskeletal tension associated osteogenesis of mouse adipose-derived stromal cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- High-throughput sequencing analysis of the expression profile of microRNAs and target genes in mechanical force-induced osteoblastic/cementoblastic differentiation of human periodontal ligament cells.American journal of translational research · 2019Article
- Nanofibers Regulate Single Bone Marrow Stem Cell Osteogenesis via FAK/RhoA/YAP1 Pathway.ACS applied materials & interfaces · 2018Article
- Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.Scientific reports · 2018Article
- miR-125a-3p and miR-483-5p promote adipogenesis via suppressing the RhoA/ROCK1/ERK1/2 pathway in multiple symmetric lipomatosis.Scientific reports · 2015Article
- Transcriptional network analysis for the regulation of left ventricular hypertrophy and microvascular remodeling.Journal of cardiovascular translational research · 2013Article
- Active RHOA favors retention of human hematopoietic stem/progenitor cells in their niche.Journal of biomedical science · 2013Article
- Comparison of Effects of Mechanical Stretching on Osteogenic Potential of ASCs and BMSCs.Bone research · 2013Article
- Adhesive and mechanical regulation of mesenchymal stem cell differentiation in human bone marrow and periosteum-derived progenitor cells.Biology open · 2012Article
- Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo.Stem cells and development · 2011Article
- Proliferation of preosteoblasts on TiORSC advancesArticle
- Mechanosensitive Non-Coding RNAs in Osteogenesis of Mesenchymal Stem Cells.Cell transplantationReview
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCytoskeletal tension is an intracellular mechanism through which cells convert a mechanical signal into a biochemical response, including production of cytokines and activation of various signaling pathways. METHODS/PRINCIPAL
findingsAdipose-derived stromal cells (ASCs) were allowed to spread into large cells by seeding them at a low-density (1,250 cells/cm(2)), which was observed to induce osteogenesis. Conversely, ASCs seeded at a high-density (25,000 cells/cm(2)) featured small cells that promoted adipogenesis. RhoA and actin filaments were altered by changes in cell size. Blocking actin polymerization by Cytochalasin D influenced cytoskeletal tension and differentiation of ASCs. To understand the potential regulatory mechanisms leading to actin cytoskeletal tension, cDNA microarray was performed on large and small ASCs. Connective tissue growth factor (CTGF) was identified as a major regulator of osteogenesis associated with RhoA mediated cytoskeletal tension. Subsequently, knock-down of CTGF by siRNA in ASCs inhibited this osteogenesis. CONCLUSIONS/SIGNIFICANCE: We conclude that CTGF is important in the regulation of cytoskeletal tension mediated ASC osteogenic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.