ArticlePloS one2010
The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- A high throughput assay for measuring secreted protein based on a de novo fluorescent reporter reveals regulatory and structural insights in Salmonella type three secretion system.Protein science : a publication of the Protein Society · 2025Article
- A Systematic Compilation of Human SH3 Domains: A Versatile Superfamily in Cellular Signaling.Cells · 2023Review
- Construction of a constitutively active type III secretion system for heterologous protein secretion.Applied microbiology and biotechnology · 2023Article
- Article
- An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system.Microbial cell factories · 2021Article
- Regulating Cdc42 and Its Signaling Pathways in Cancer: Small Molecules and MicroRNA as New Treatment Candidates.Molecules (Basel, Switzerland) · 2018Review
- Intramolecular interactions between the Dbl homology (DH) domain and the carboxyl-terminal region of myosin II-interacting guanine nucleotide exchange factor (MyoGEF) act as an autoinhibitory mechanism for the regulation of MyoGEF functions.The Journal of biological chemistry · 2014Article
- Using transcriptional control to increase titers of secreted heterologous proteins by the type III secretion system.Applied and environmental microbiology · 2014Article
- Emerging roles for intersectin (ITSN) in regulating signaling and disease pathways.International journal of molecular sciences · 2013Review
- Structural basis for autoinhibition of the guanine nucleotide exchange factor FARP2.Structure (London, England : 1993) · 2013Article
- Small molecule targeting Cdc42-intersectin interaction disrupts Golgi organization and suppresses cell motility.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Structure of the Rho-specific guanine nucleotide-exchange factor Xpln.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Deconstructing signal transduction pathways that regulate the actin cytoskeleton in dendritic spines.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Autoinhibition of GEF activity in Intersectin 1 is mediated by the short SH3-DH domain linker.Protein science : a publication of the Protein Society · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Intersectin-1L is a member of the Dbl homology (DH) domain guanine nucleotide exchange factors (GEF) which control Rho-family GTPase signaling. Intersectin-1L is a GEF that is specific for Cdc42. It plays an important role in endocytosis, and is regulated by several partners including the actin regulator N-WASP. Intact intersectin-1L shows low Cdc42 exchange activity, although the isolated catalytic DH domain shows high activity. This finding suggests that the molecule is autoinhibited. To investigate the mechanism of autoinhibition we have constructed a series of domain deletions. We find that the five SH3 domains of intersectin are important for autoinhibition, with the fifth domain (SH3(E)) being sufficient for the bulk of the autoinhibitory effect. This SH3 domain appears to primarily interact with the DH domain. We have determined the crystal structure of the SH3(E)-DH domain construct, which shows a domain swapped arrangement in which the SH3 from one monomer interacts with the DH domain of the other monomer. Analytical ultracentrifugation and gel filtration, however, show that under biochemical concentrations, the construct is fully monomeric. Thus we propose that the actual autoinhibited structure contains the related intramolecular SH3(E)-DH interaction. We propose a model in which this intramolecular interaction may block or distort the GTPase binding region of the DH domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.