ArticlePloS one2010
Protective unfolded protein response in human pancreatic beta cells transplanted into mice.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Trial
- Importance of unfolded protein response modulation on diabetes management: a systematic review.Journal of diabetes and metabolic disorders · 2024Review
- Harmine and exendin-4 combination therapy safely expands human β cell mass in vivo in a mouse xenograft system.Science translational medicine · 2024Article
- Methylation and Expression ofNutrients · 2021Article
- Lower mean blood glucose during short-term intensive insulin therapy is associated with long-term glycemic remission in patients with newly diagnosed type 2 diabetes: Evidence-based recommendations for standardization.Journal of diabetes investigation · 2018Article
- Metabolomics Study of the Effects of Inflammation, Hypoxia, and High Glucose on Isolated Human Pancreatic Islets.Journal of proteome research · 2017Article
- Glial cell-line derived neurotrophic factor protects human islets from nutrient deprivation and endoplasmic reticulum stress induced apoptosis.Scientific reports · 2017Article
- Evidence of stress in β cells obtained with laser capture microdissection from pancreases of brain dead donors.Islets · 2017Article
- Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas.Journal of physiology and biochemistry · 2016Article
- Article
- The proapoptotic BH3-only proteins Bim and Puma are downstream of endoplasmic reticulum and mitochondrial oxidative stress in pancreatic islets in response to glucotoxicity.Cell death & disease · 2014Article
- ER Stress and β-Cell Pathogenesis of Type 1 and Type 2 Diabetes and Islet Transplantation.Cell medicine · 2013Review
- Islet β cell mass in diabetes and how it relates to function, birth, and death.Annals of the New York Academy of Sciences · 2013Review
- Laser capture microdissection for protein and NanoString RNA analysis.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Hepatic nuclear factor 1alpha (HNF1alpha) dysfunction down-regulates X-box-binding protein 1 (XBP1) and sensitizes beta-cells to endoplasmic reticulum stress.The Journal of biological chemistry · 2011Article
- Severity of diabetes governs vascular lipoprotein lipase by affecting enzyme dimerization and disassembly.Diabetes · 2011Article
- Endoplasmic reticulum stress and pancreatic β-cell death.Trends in endocrinology and metabolism: TEM · 2011Review
- β-Cell failure in type 2 diabetes.Journal of diabetes investigation · 2011Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThere is great interest about the possible contribution of ER stress to the apoptosis of pancreatic beta cells in the diabetic state and with islet transplantation. METHODS AND
findingsExpression of genes involved in ER stress were examined in beta cell enriched tissue obtained with laser capture microdissection (LCM) from frozen sections of pancreases obtained from non-diabetic subjects at surgery and from human islets transplanted into ICR-SCID mice for 4 wk. Because mice have higher glucose levels than humans, the transplanted beta cells were exposed to mild hyperglycemia and the abnormal environment of the transplant site. RNA was extracted from the LCM specimens, amplified and then subjected to microarray analysis. The transplanted beta cells showed an unfolded protein response (UPR). There was activation of many genes of the IRE-1 pathway that provide protection against the deleterious effects of ER stress, increased expression of ER chaperones and ERAD (ER-associated protein degradation) proteins. The other two arms of ER stress, PERK and ATF-6, had many down regulated genes. Downregulation of EIF2A could protect by inhibiting protein synthesis. Two genes known to contribute to apoptosis, CHOP and JNK, were downregulated.
conclusionsHuman beta cells in a transplant site had UPR changes in gene expression that protect against the proapoptotic effects of unfolded proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.