ArticleMolecular and cellular biology2010
Dynamic localization of Fus3 mitogen-activated protein kinase is necessary to evoke appropriate responses and avoid cytotoxic effects.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Constitutively active RAS prolongs Cdc42 signalling, while MAPK signalling is attenuated during fission yeast mating.PLoS genetics · 2026Article
- Single-particle imaging of stress-promoters induction reveals the interplay between MAPK signaling, chromatin and transcription factors.Nature communications · 2020Article
- Article
- Quantitative analysis of the yeast pheromone pathway.Yeast (Chichester, England) · 2019Article
- Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion.Journal of cell science · 2018Article
- Stress-induced nuclear accumulation is dispensable for Hog1-dependent gene expression and virulence in a fungal pathogen.Scientific reports · 2017Article
- Nuclear relocation of Kss1 contributes to the specificity of the mating response.Scientific reports · 2017Article
- Selection and stabilization of endocytic sites by Ede1, a yeast functional homologue of human Eps15.Molecular biology of the cell · 2017Article
- Single-cell dynamics and variability of MAPK activity in a yeast differentiation pathway.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Formation of subnuclear foci is a unique spatial behavior of mating MAPKs during hyperosmotic stress.Cell reports · 2013Article
- Single-cell analysis reveals that insulation maintains signaling specificity between two yeast MAPK pathways with common components.Science signaling · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cellular responses to many external stimuli are mediated by mitogen-activated protein kinases (MAPKs). We investigated whether dynamic intracellular movement contributes to the spatial and temporal characteristics of the responses elicited by a prototypic MAPK, Fus3, in the mating pheromone response pathway in budding yeast (Saccharomyces cerevisiae). Confining Fus3 in the nucleus, via fusion to a histone H2B, reduced MAPK activation and diminished all responses (pheromone-induced gene expression, cell cycle arrest, projection formation, and mating). Elimination of MAPK phosphatases restored more robust outputs for all responses, indicating that nuclear sequestration impedes full MAPK activation but does not abrogate its functional competence. Restricting Fus3 to the plasma membrane, via fusion to a lipid-modified CCaaX motif, led to MAPK hyperactivation yet severely impaired all response outputs. Fus3-CCaaX also caused aberrant cell morphology and a proliferation defect. Unlike similar phenotypes induced by pathway hyperactivation via upstream components, these deleterious effects were independent of the downstream transcription factor Ste12. Thus, appropriate cellular responses require free subcellular MAPK transit to disseminate MAPK activity optimally because preventing dynamic MAPK movement either markedly impaired signal-dependent activation and/or resulted in improper biological outputs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.