ArticleInfection and immunity2010
Transcellular passage of Neisseria meningitidis across a polarized respiratory epithelium.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Article
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- Impact of host factors and invasive meningococci on bacterial adhesion, proliferation, primary nasal epithelial barrier function, and immune response.Microbiology spectrum · 2025Article
- Internalization ofMicroorganisms · 2025Article
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- Interaction ofFrontiers in cellular and infection microbiology · 2024Article
- Detection of Novel US Neisseria meningitidis Urethritis Clade Subtypes in Japan.Emerging infectious diseases · 2023Review
- Variable disruption of epithelial monolayers byMicrobiology (Reading, England) · 2023Article
- Multilayer Regulation ofMicroorganisms · 2022Article
- The Host-Pathogen Interactions and Epicellular Lifestyle ofFrontiers in cellular and infection microbiology · 2022Review
- Interactions and Signal Transduction Pathways Involved during Central Nervous System Entry byInternational journal of molecular sciences · 2020Review
- Article
- Molecular interactions between Neisseria meningitidis and its human host.Cellular microbiology · 2019Review
- Pathogenic Mannheimia haemolytica Invades Differentiated Bovine Airway Epithelial Cells.Infection and immunity · 2019Article
- Super-Resolution Microscopy Reveals Local Accumulation of Plasma Membrane Gangliosides atFrontiers in cell and developmental biology · 2019Article
- Neisseria Heparin Binding Antigen is targeted by the human alternative pathway C3-convertase.PloS one · 2018Article
- Targeting the Mucosal Barrier: How Pathogens Modulate the Cellular Polarity Network.Cold Spring Harbor perspectives in biology · 2017Review
- Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis.Scientific reports · 2017Article
- Of the Phrensy: an update on the epidemiology and pathogenesis of bacterial meningitis in the pediatric population.F1000Research · 2017Review
- UnravelingF1000Research · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neisseria meningitidis is a major cause of sepsis and meningitis but is also a common commensal, present in the nasopharynx of between 8 and 20% of healthy individuals. During carriage, the bacterium is found on the surface of the nasopharyngeal epithelium and in deeper tissues, while to develop disease the meningococcus must spread across the respiratory epithelium and enter the systemic circulation. Therefore, investigating the pathways by which N. meningitidis crosses the epithelial barrier is relevant for understanding carriage and disease but has been hindered by the lack of appropriate models. Here, we have established a physiologically relevant model of the upper respiratory epithelial cell barrier to investigate the mechanisms responsible for traversal of N. meningitidis. Calu-3 human respiratory epithelial cells were grown on permeable cell culture membranes to form polarized monolayers of cells joined by tight junctions. We show that the meningococcus crosses the epithelial cell barrier by a transcellular route; traversal of the layer did not disrupt its integrity, and bacteria were detected within the cells of the monolayer. We demonstrate that successful traversal of the epithelial cell barrier by N. meningitidis requires expression of its type 4 pili (Tfp) and capsule and is dependent on the host cell microtubule network. The Calu-3 model should be suitable for dissecting the pathogenesis of infections caused by other respiratory pathogens, as well as the meningococcus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.