ArticleRNA (New York, N.Y.)2010
Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-end.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Light Scattering Technology-Combined Ligation-Dependent Loop-Mediated Isothermal Amplification (LL-LAMP) for Sensitive Detection of RNA.ACS omega · 2022Article
- Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection.RNA (New York, N.Y.) · 2019Article
- RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection.Scientific reports · 2018Article
- Limited reverse transcriptase activity of phi29 DNA polymerase.Nucleic acids research · 2018Article
- Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA.Scientific reports · 2017Article
- Highly specific imaging of mRNA in single cells by target RNA-initiated rolling circle amplification.Chemical science · 2017Article
- Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation.RNA (New York, N.Y.) · 2017Article
- Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues.Nature protocols · 2015Article
- Article
- Cleavage-based signal amplification of RNA.Nature communications · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We improved the target RNA-primed RCA technique for direct detection and analysis of RNA in vitro and in situ. Previously we showed that the 3' --> 5' single-stranded RNA exonucleolytic activity of Phi29 DNA polymerase converts the target RNA into a primer and uses it for RCA initiation. However, in some cases, the single-stranded RNA exoribonucleolytic activity of the polymerase is hindered by strong double-stranded structures at the 3'-end of target RNAs. We demonstrate that in such hampered cases, the double-stranded RNA-specific Escherichia coli RNase III efficiently assists Phi29 DNA polymerase in converting the target RNA into a primer. These observations extend the target RNA-primed RCA possibilities to test RNA sequences distanced far from the 3'-end and customize this technique for the inner RNA sequence analysis.
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