ArticleACS chemical biology2010
Multicolor monitoring of dysregulated protein kinases in chronic myelogenous leukemia.
Article in ACS chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- A Triangulenum-Based Biosensor Platform for Mapping Kinase Activity in T Cells.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Shining Light on Protein Kinase Biomarkers with Fluorescent Peptide Biosensors.Life (Basel, Switzerland) · 2022Review
- Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Assays for tyrosine phosphorylation in human cells.Methods in enzymology · 2019Article
- A Role for the Non-Receptor Tyrosine Kinase Abl2/Arg in Experimental Neuroinflammation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2018Article
- Kinase-templated abiotic reaction.Chemical science · 2017Article
- BIRC6 mediates imatinib resistance independently of Mcl-1.PloS one · 2017Article
- Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.Proteomes · 2015Review
- Multicolored, Tb³⁺-Based Antibody-Free Detection of Multiple Tyrosine Kinase Activities.Analytical chemistry · 2015Article
- Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells.Angewandte Chemie (International ed. in English) · 2013Article
- Monitoring of protein arginine deiminase activity by using fluorescence quenching: multicolor visualization of citrullination.Angewandte Chemie (International ed. in English) · 2013Article
- Application of multiplexed kinase inhibitor beads to study kinome adaptations in drug-resistant leukemia.PloS one · 2013Article
- A peptide photoaffinity probe specific for the active conformation of the Abl tyrosine kinase.Chembiochem : a European journal of chemical biology · 2012Article
- Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS.Molecular bioSystems · 2012Article
- Metabolism of peptide reporters in cell lysates and single cells.The Analyst · 2012Article
- Interrogating signaling nodes involved in cellular transformations using kinase activity probes.Chemistry & biology · 2012Article
- SRC kinase regulation in progressively invasive cancer.PloS one · 2012Article
- Measuring enzyme activity in single cells.Trends in biotechnology · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Bcr-Abl and Lyn protein tyrosine kinases have been separately linked to the emergence of imatinib resistance in patients with chronic myelogenous leukemia. We have developed fluorescent sensors for these kinases that are enzymatically and photophysically distinct, allowing us to simultaneously, yet separately, visualize the tyrosine kinase activities of both Abl and Lyn. Multicolor monitoring revealed that an imatinib-resistant cell line (MYL-R) displays a remarkable 13-fold enhancement in Lyn kinase activity relative to its imatinib-sensitive counterpart (MYL). By contrast, both cell lines display nearly identical Abl activities. The upregulation of Lyn kinase phosphotransferase activity in MYL-R cells is linked to an overexpression of the Lyn B isoform. Furthermore, MYL-R cells possess a 4-fold higher level of activated Lyn and 5-fold lower level of autoinhibited Lyn than MYL cells. Finally, studies with an activating SH2 ligand revealed that Lyn from imatinib-resistant MYL-R cells is primed and active, whereas Lyn from imatinib-sensitive cells is dependent upon phosphorylated SH2 ligands for activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.