ArticleJournal of the American Chemical Society2010
An array-based method to identify multivalent inhibitors.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 87 citations in OpenAlex.
- GCI-Based Affinity Screening of Synthetic Oligomannosides toward Concanavalin A.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Molecularly Defined Glycocalyx Models Reveal ABJACS Au · 2025Article
- Exploring the Conformational Changes and Dynamics of Mucins in Their Free State and in Complex with Mucin-Binding Proteins Using NMR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Sugar-Coated: Can Multivalent Glycoconjugates Improve upon Nature's Design?Journal of the American Chemical Society · 2024Review
- Sub-10 μm Soft Interlayers Integrating Patterned Multivalent Biomolecular Binding Environments.ACS applied materials & interfaces · 2024Article
- Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Human oral lectin ZG16B acts as a cell wall polysaccharide probe to decode host-microbe interactions with oral commensals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Surface-Templated Glycopolymer Nanopatterns Transferred to Hydrogels for Designed Multivalent Carbohydrate-Lectin Interactions across Length Scales.Journal of the American Chemical Society · 2023Article
- GlyNet: a multi-task neural network for predicting protein-glycan interactions.Chemical science · 2022Article
- An Automated Approach to Assess Relative Galectin-Glycan Affinity Following Glycan Microarray Analysis.Frontiers in molecular biosciences · 2022Article
- Synthetic Plant Glycan Microarrays as Tools for Plant Biology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage.Nature chemical biology · 2021Article
- Enhanced Binding and Reduced Immunogenicity of Glycoconjugates Prepared via Solid-State Photoactivation of Aliphatic Diazirine Carbohydrates.Bioconjugate chemistry · 2021Article
- Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021Article
- On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems.ChemistryOpen · 2020Review
- Multivalent binding of concanavalin A on variable-density mannoside microarrays.Faraday discussions · 2019Article
- Factors contributing to variability of glycan microarray binding profiles.Faraday discussions · 2019Article
- Synthetic glycoscapes: addressing the structural and functional complexity of the glycocalyx.Interface focus · 2019Review
- Efficient Synthesis of Heparinoid Bioconjugates for Tailoring FGF2 Activity at the Stem Cell-Matrix Interface.Bioconjugate chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Carbohydrate-protein interactions play a critical role in a variety of biological processes, and agonists/antagonists of these interactions are useful as biological probes and therapeutic agents. Most carbohydrate-binding proteins achieve tight binding through formation of a multivalent complex. Therefore, both ligand structure and presentation contribute to recognition. Since there are many potential combinations of structure, spacing, and orientation to consider and the optimal one cannot be predicted, high-throughput approaches for analyzing carbohydrate-protein interactions and designing inhibitors are appealing. In this report, we develop a strategy to vary neoglycoprotein density on a surface of a glycan array. This feature of presentation was combined with variations in glycan structure and glycan density to produce an array with approximately 600 combinations of glycan structure and presentation. The unique array platform allows one to distinguish between different types of multivalent complexes on the array surface. To illustrate the advantages of this format, it was used to rapidly identify multivalent probes for various lectins. The new array was first tested with several plant lectins, including concanavalin A (conA), Vicia villosa isolectin B4 (VVL-B(4)), and Ricinus communis agglutinin (RCA120). Next, it was used to rapidly identify potent multivalent inhibitors of Pseudomonas aeruginosa lectin I (PA-IL), a key protein involved in opportunistic infections of P. aeruginosa , and mouse macrophage galactose-type lectin (mMGL-2), a protein expressed on antigen presenting cells that may be useful as a vaccine targeting receptor. An advantage of the approach is that structural information about the lectin/receptor is not required to obtain a multivalent inhibitor/probe.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.