ArticleCellular and molecular life sciences : CMLS2010
SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum.
Article in Cellular and molecular life sciences : CMLS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- TKL3 regulates blood-stage fitness, male gamete fertility, and transmission-stage development in Plasmodium berghei.PLoS pathogens · 2026Article
- Article
- TKL family kinases in human apicomplexan pathogens.Molecular and biochemical parasitology · 2024Review
- Tyrosine Kinase Inhibitors Display Potent Activity against Cryptosporidium parvum.Microbiology spectrum · 2023Article
- A framework for signaling throughout the life cycle of Babesia species.Molecular microbiology · 2021Review
- Review
- Inhibition of Resistance-Refractory P. falciparum Kinase PKG Delivers Prophylactic, Blood Stage, and Transmission-Blocking Antiplasmodial Activity.Cell chemical biology · 2020Article
- Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA.PLoS biology · 2019Article
- Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported to host erythrocytes.Scientific reports · 2019Article
- Genome-Wide Identification and Evolutionary Analysis of Sarcocystis neurona Protein Kinases.Pathogens (Basel, Switzerland) · 2017Article
- Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role for PfDHHC9 in gametocytogenesis.Cellular microbiology · 2016Article
- Enzyme Activity Assays for Protein Kinases: Strategies to Identify Active Substrates.Current drug discovery technologies · 2016Review
- In silico prediction of antimalarial drug target candidates.International journal for parasitology. Drugs and drug resistance · 2012Article
- Toxoplasma and Plasmodium protein kinases: roles in invasion and host cell remodelling.International journal for parasitology · 2012Review
- Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum.Nature communications · 2011Article
- Plasmodium falciparum NIMA-related kinase Pfnek-1: sex specificity and assessment of essentiality for the erythrocytic asexual cycle.Microbiology (Reading, England) · 2011Article
- The Cryptosporidium parvum kinome.BMC genomics · 2011Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist responsible for the most virulent form of human malaria, remain unknown. Here we present a thorough characterisation of PfTKL3 (PF13_0258), an enzyme that belongs to the tyrosine kinase-like kinase (TKL) group. We demonstrate by reverse genetics that PfTKL3 is essential for asexual parasite proliferation in human erythrocytes. PfTKL3 is expressed in both asexual and gametocytes stages, and in the latter the protein co-localises with cytoskeleton microtubules. Recombinant PfTKL3 displays in vitro autophosphorylation activity and is able to phosphorylate exogenous substrates, and both activities are dramatically dependent on the presence of an N-terminal "sterile alpha-motif" domain. This study identifies PfTKL3 as a validated drug target amenable to high-throughput screening.
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