ArticleTransgenic research2011
Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Review
- Strategies for Efficient RNAi-Based Gene Silencing of Viral Genes for Disease Resistance in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum.Scientific reports · 2021Article
- Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion.Transgenic research · 2020Article
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Review
- Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing.BMC plant biology · 2018Article
- Generation of marker-free transgenic plants concurrently resistant to a DNA geminivirus and a RNA tospovirus.Scientific reports · 2014Article
- Engineered resistance in potato against potato leafroll virus, potato virus A and potato virus Y.Virus genes · 2013Article
- Transgenerational maintenance of transgene body CG but not CHG and CHH methylation.Epigenetics · 2012Article
- Article
- Resistance to multiple viruses in transgenic tobacco expressing fused, tandem repeat, virus-derived double-stranded RNAs.Virus genes · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we had shown that stable expression of a hairpin RNA sharing homology with the coat protein (CP) of the Cucumber mosaic virus (CMV) (hpRNA(CMV)) produced CMV resistant Nicotiana tabacum plants. However, only 17% of the hpRNA(CMV)-expressing plants generated substantial amounts of siRNAs that mediated CMV resistance (siRNAs(CMV)). Here, we demonstrate that the transcription of a hpRNA(CMV) per se is not sufficient to trigger cytoplasmic and nuclear RNAi. A multiple-transgene copy line showed a strong resistance phenotype. Segregation of individual copies revealed that in one locus, the transgene-produced hpRNA(CMV) transcript was processed into 21-nt and 24-nt siRNAs(CMV) and lines containing this locus were resistant. At a second locus, where the transgene was shown to be transcribed, no siRNAs(CMV) were produced and lines harbouring only this locus were susceptible. In addition, the second locus failed to trigger de novo RNA-directed DNA methylation (RdDM) in cis, of its cognate sequence. However, after being induced in trans, methylation in the transcribed region of the transgene was maintained in both CG and CHG residues. Sequence-specific maintenance of methylation in transcribed regions, as well as diverse RNA degradation pathways in plants are discussed in view of our observations.
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