ArticlePlanta2010
Host-derived suppression of nematode reproductive and fitness genes decreases fecundity of Heterodera glycines Ichinohe.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Biochemical and nanotechnological approaches to combat phytoparasitic nematodes.Plant biotechnology journal · 2024Review
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression.Planta · 2022Article
- Host-induced silencing of a nematode chitin synthase gene enhances resistance of soybeans to both pathogenic Heterodera glycines and Fusarium oxysporum.Plant biotechnology journal · 2022Article
- Enhanced resistance to soybean cyst nematode in transgenic soybean via host-induced silencing of vital Heterodera glycines genes.Transgenic research · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- RNA Interference for Improving Disease Resistance in Plants and Its Relevance in This Clustered Regularly Interspaced Short Palindromic Repeats-Dominated Era in Terms of dsRNA-Based Biopesticides.Frontiers in plant science · 2022Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Differential Morphological, Structural and Biological Characteristics of Cysts inThe plant pathology journal · 2020Article
- Chitin Biosynthesis Inhibition ofInternational journal of molecular sciences · 2020Article
- Host-derived gene silencing of parasite fitness genes improves resistance to soybean cyst nematodes in stable transgenic soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Discovery and profiling of small RNAs from Puccinia triticina by deep sequencing and identification of their potential targets in wheat.Functional & integrative genomics · 2019Article
- Rational design of biosafe crop resistance to a range of nematodes using RNA interference.Plant biotechnology journal · 2018Article
- Genome-wide identification of soybean microRNA responsive to soybean cyst nematodes infection by deep sequencing.BMC genomics · 2017Article
- RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes.Frontiers in plant science · 2017Review
- Article
- Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection.Nature plants · 2016Article
- Identification and functional analysis of secreted effectors from phytoparasitic nematodes.BMC microbiology · 2016Review
- Fighting Asian Soybean Rust.Frontiers in plant science · 2016Review
- Integrated signaling networks in plant responses to sedentary endoparasitic nematodes: a perspective.Plant cell reports · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To control Heterodera glycines Ichinohe (soybean cyst nematode) in Glycine max (L.) Merr. (soybean), we evaluated the use of producing transgenic soybean seedlings expressing small interfering RNAs (siRNAs) against specific H. glycines genes. Gene fragments of three genes related to nematode reproduction or fitness (Cpn-1, Y25 and Prp-17) were PCR-amplified using specific primers and independently cloned into the pANDA35HK RNAi vector using a Gateway cloning strategy. Soybean roots were transformed with these constructions using a composite plant system. Confirmation of transformation was attained by PCR and Southern blot analysis. Transgene expression was detected using reverse transcription PCR (RT-PCR) and expression of siRNAs was confirmed in transgenic plants using northern blot analysis. Bioassays performed on transgenic composite plants expressing double-stranded RNA fragments of Cpn-1, Y25 and Prp-17 genes resulted in a 95, 81 and 79% reduction for eggs g(-1) root, respectively. Furthermore, we demonstrated a significant reduction in transcript levels of the Y25 and Prp-17 genes of the nematodes feeding on the transgenic roots via real-time RT-PCR whereas the expression of non-target genes were not affected. The results of this study demonstrate that over-expression of RNA interference constructs of nematode reproduction or fitness-related genes can effectively control H. glycines infection with levels of suppression comparable to conventional resistance.
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Registered trials
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