ArticleNature cell biology2010
Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions.
Article in Nature cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 211 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
211 citing papers in PubMed, 458 citations in OpenAlex.
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- How the Fibrotic Lung Microenvironment Regulates Stem Cell Behavior: Immune Cues, Extracellular Matrix Remodeling, and Therapeutic Implications.Analytical cellular pathology (Amsterdam) · 2026Review
- Heterogeneity as a feature: unraveling chromatin's role in nuclear mechanics.Nucleus (Austin, Tex.) · 2025Review
- Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes.Journal of cell science · 2025Article
- Differential roles of cyclin-CDK1 complexes in cell migration and invasion.Journal of cell science · 2025Article
- Long-term expansion of basal cells and the novel differentiation methods identify mechanisms for switching Claudin expression in normal epithelia.Scientific reports · 2025Article
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- FAK mediates mechanical signaling to maintain epithelial homeostasis through YAP/TAZ-TEADs.Histochemistry and cell biology · 2025Article
- Influence of cell shape on sonoporation efficiency in microbubble-facilitated delivery using micropatterned cell arrays.Scientific reports · 2024Article
- Advances in micropatterning technology for mechanotransduction research.Mechanobiology in medicine · 2024Review
- Matrix stiffness-dependent regulation of immunomodulatory genes in human MSCs is associated with the lncRNA CYTOR.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ontogeny of Skin Stem Cells and Molecular Underpinnings.Current issues in molecular biology · 2024Review
- SRF transcriptionally regulates the oligodendrocyte cytoskeleton during CNS myelination.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural anisotropy results in mechano-directional transport of proteins across nuclear pores.Nature physics · 2024Article
- The first embryo, the origin of cancer and animal phylogeny. II. The neoplastic process as an evolutionary engine.Journal of biosciences · 2024Article
- Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability.Stem cells (Dayton, Ohio) · 2023Article
- 3D micropattern force triggers YAP nuclear entry by transport across nuclear pores and modulates stem cells paracrine.National science review · 2023Article
- Laser Micropatterning Promotes Rete Ridge Formation and Enhanced Engineered Skin Strength without Increased Inflammation.Bioengineering (Basel, Switzerland) · 2023Article
- A guide to functionalisation and bioconjugation strategies to surface-initiated polymer brushes.Chemical communications (Cambridge, England) · 2023Review
151 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Epidermal homeostasis depends on a balance between stem cell renewal and differentiation and is regulated by extrinsic signals from the extracellular matrix (ECM). A powerful approach to analysing the pathways involved is to engineer single-cell microenvironments in which individual variables are precisely and quantitatively controlled. Here, we employ micropatterned surfaces to identify the signalling pathways by which restricted ECM contact triggers human epidermal stem cells to initiate terminal differentiation. On small (20 microm diameter) circular islands, keratinocytes remained rounded, and differentiated at higher frequency than cells that could spread on large (50 microm diameter) islands. Differentiation did not depend on ECM composition or density. Rather, the actin cytoskeleton mediated shape-induced differentiation by regulating serum response factor (SRF) transcriptional activity. Knockdown of SRF or its co-factor MAL inhibited differentiation, whereas overexpression of MAL stimulated SRF activity and involucrin expression. SRF target genes FOS and JUNB were also required for differentiation: c-Fos mediated serum responsiveness, whereas JunB was regulated by actin and MAL. Our findings demonstrate how biophysical cues are transduced into transcriptional responses that determine epidermal cell fate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.