ArticleThe Journal of experimental biology2010
Controlling anoxic tolerance in adult Drosophila via the cGMP-PKG pathway.
Article in The Journal of experimental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Anticonvulsant effects of novel and repurposed drugs on docetaxel-induced neuropathy in C. elegans.PloS one · 2026Article
- Low Glycolysis Is Neuroprotective during Anoxic Spreading Depolarization (SD) and Reoxygenation in Locusts.eNeuro · 2023Article
- Knockdown of a Cyclic Nucleotide-Gated Ion Channel Impairs Locomotor Activity and Recovery From Hypoxia in AdultFrontiers in physiology · 2022Article
- TheJournal of neurogenetics · 2021Article
- Expression of theJournal of neurogenetics · 2021Article
- TheInternational journal of environmental research and public health · 2021Article
- Neural shutdown under stress: an evolutionary perspective on spreading depolarization.Journal of neurophysiology · 2020Review
- Genome-Wide Association Analysis of Anoxia Tolerance inG3 (Bethesda, Md.) · 2019Article
- egl-4 modulates electroconvulsive seizure duration in C. elegans.Invertebrate neuroscience : IN · 2018Article
- The DrosophilaCognitive development · 2017Article
- 'Foraging' for a place to lay eggs: A genetic link between foraging behaviour and oviposition preferences.PloS one · 2017Article
- White - cGMP Interaction Promotes Fast Locomotor Recovery from Anoxia in Adult Drosophila.PloS one · 2017Article
- Spreading depolarization in the brain of Drosophila is induced by inhibition of the Na+/K+-ATPase and mitigated by a decrease in activity of protein kinase G.Journal of neurophysiology · 2016Article
- Octopamine stabilizes conduction reliability of an unmyelinated axon during hypoxic stress.Journal of neurophysiology · 2016Article
- Mechanisms of spreading depolarization in vertebrate and insect central nervous systems.Journal of neurophysiology · 2016Review
- Article
- Stimulation of the cardiac myocyte Na+-K+ pump due to reversal of its constitutive oxidative inhibition.American journal of physiology. Cell physiology · 2015Article
- Pushing the limit: examining factors that affect anoxia tolerance in a single genotype of adult D. melanogaster.Scientific reports · 2015Article
- Na+-K+-ATPase trafficking induced by heat shock pretreatment correlates with increased resistance to anoxia in locusts.Journal of neurophysiology · 2014Article
- Anaerobic metabolism at thermal extremes: a metabolomic test of the oxygen limitation hypothesis in an aquatic insect.Integrative and comparative biology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
In this study we identify a cGMP-dependent protein kinase (PKG) cascade as a biochemical pathway critical for controlling low-oxygen tolerance in the adult fruit fly, Drosophila melanogaster. Even though adult Drosophila can survive in 0% oxygen (anoxia) environments for hours, air with less than 2% oxygen rapidly induces locomotory failure resulting in an anoxic coma. We use natural genetic variation and an induced mutation in the foraging (for) gene, which encodes a Drosophila PKG, to demonstrate that the onset of anoxic coma is correlated with PKG activity. Flies that have lower PKG activity demonstrate a significant increase in time to the onset of anoxic coma. Further, in vivo pharmacological manipulations reveal that reducing either PKG or protein phosphatase 2A (PP2A) activity increases tolerance of behavior to acute hypoxic conditions. Alternatively, PKG activation and phosphodiesterase (PDE5/6) inhibition significantly reduce the time to the onset of anoxic coma. By manipulating these targets in paired combinations, we characterized a specific PKG cascade, with upstream and downstream components. Further, using genetic variants of PKG expression/activity subjected to chronic anoxia over 6 h, approximately 50% of animals with higher PKG activity survive, while only approximately 25% of those with lower PKG activity survive after a 24 h recovery. Therefore, in this report we describe the PKG pathway and the differential protection of function vs survival in a critically low oxygen environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.