ArticleJournal of bacteriology2010
Identification of a receptor subunit and putative ligand-binding residues involved in the Bacillus megaterium QM B1551 spore germination response to glucose.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Genetic Suppression Meets Structure Prediction: Probing a Spore Germination Receptor Complex.Journal of bacteriology · 2022Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Effects ofAnimals : an open access journal from MDPI · 2021Article
- Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.Journal of bacteriology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.Scientific reports · 2018Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- Surviving Between Hosts: Sporulation and Transmission.Microbiology spectrum · 2016Review
- The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores.Applied and environmental microbiology · 2016Article
- Draft Genome Sequence of Bacillus megaterium BHG1.1, a Strain Isolated from Bar-Headed Goose (Anser indicus) Feces on the Qinghai-Tibet Plateau.Genome announcements · 2016Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
- Germination of spores of Bacillus species: what we know and do not know.Journal of bacteriology · 2014Review
- Identification of new proteins that modulate the germination of spores of bacillus species.Journal of bacteriology · 2013Article
- Investigating the functional hierarchy of Bacillus megaterium PV361 spore germinant receptors.Journal of bacteriology · 2013Article
- Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.Journal of bacteriology · 2013Article
- Role of the gerA operon in L-alanine germination of Bacillus licheniformis spores.BMC microbiology · 2012Article
- Membrane topology of the Bacillus anthracis GerH germinant receptor proteins.Journal of bacteriology · 2012Article
- Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.Journal of bacteriology · 2011Article
- Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
The molecular basis for the recognition of glucose as a germinant molecule by spores of Bacillus megaterium QM B1551 has been examined. A chromosome-located locus (BMQ_1820, renamed gerWB) is shown to encode a receptor B-protein subunit that interacts with the GerUA and GerUC proteins to form a receptor that is cognate for both glucose and leucine. GerWB represents the third receptor B protein that binds to glucose in this strain. Site-directed mutagenesis (SDM) experiments conducted on charged proline and aromatic residues predicted to reside in the transmembrane domains of a previously characterized receptor B protein, GerVB, reveal the importance to receptor function of a cluster of residues predicted to reside in the middle of the transmembrane 6 (TM6) domain. Reductions in the region of 70- to 165-fold in the apparent affinity of receptors for glucose in which Glu196, Tyr191, and Phe192 are individually replaced by SDM indicate that some or all of these residues may be directly involved in the binding of glucose and perhaps other germinants to the germinant receptor.
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