ArticleApplied and environmental microbiology2010
Detection of Vibrio cholerae O1 and O139 in environmental water samples by an immunofluorescent-aggregation assay.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Marine bacteriamBio · 2024Article
- An efficient DNAzyme for the fluorescence detection ofFood science & nutrition · 2023Article
- Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance.Archives of microbiology · 2022Review
- Wastewater based environmental surveillance of toxigenic Vibrio cholerae in Pakistan.PloS one · 2021Article
- Particle Diffusometry: An Optical Detection Method for Vibrio cholerae Presence in Environmental Water Samples.Scientific reports · 2019Article
- Environmental Surveillance ofFrontiers in microbiology · 2018Article
- Article
- Detection of Vibrio cholerae O1 and O139 in environmental waters of rural Bangladesh: a flow-cytometry-based field trial.Epidemiology and infection · 2015Article
- A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.Applied and environmental microbiology · 2015Article
- Article
- Behavioural factors associated with diarrhea among adults over 18 years of age in Beijing, China.BMC public health · 2014Article
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental waters are an important reservoir for Vibrio cholerae, and effective surveillance of the pathogen can help to warn of and prevent infection with this potentially fatal pathogen. An immunofluorescent-aggregation (IFAG) assay to detect V. cholerae O1 and O139 was established and evaluated with estuarine water samples. The practical application of this assay was compared with the conventional culture method and real-time PCR. The IFAG method had a sensitivity of 10(3) CFU/ml for detection of V. cholerae O1 and O139 strains in a suspension containing 10 different species of enterobacterial strains (total, 10(5) CFU/ml). Ten fluorescent bacterial aggregate colonies were randomly picked and tested positive in serum agglutination tests for the V. cholerae O1 and O139 strains, showing a high specificity. The enrichment broths of 146 samples of estuarine water were tested, and the percentage positive by the IFAG assay was 19.9% (29/146), which was significantly higher than that of the conventional culture method (10.3%, 15/146; P < 0.01) but lower than that of real-time PCR (29.5%, 43/146; P < 0.01). The coincidence rates of real-time PCR and IFAG detection were decreased with the reduction of the V. cholerae concentration. The IFAG method, with a high specificity and a relatively high sensitivity, may be used for detection and isolation of V. cholerae in environmental water samples.
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