ArticleApplied and environmental microbiology2010
Rapid microarray-based genotyping of enterohemorrhagic Escherichia coli serotype O156:H25/H-/Hnt isolates from cattle and clonal relationship analysis.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- Genomic characterization and therapeutic potential of five broad-spectrum lytic bacteriophages against multidrug-resistant avian pathogenicMicrobiology spectrum · 2026Article
- Diversity of third-generation cephalosporin-resistant enterobacterales from human and animal samples obtained in Owerri, Nigeria.BMC infectious diseases · 2026Article
- Presence of β-Lactamase-producing Enterobacterales andInternational journal of molecular sciences · 2021Article
- Molecular characterisation of extended-spectrum ß-lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria.BMC veterinary research · 2021Article
- Article
- Metabolic Traits of Bovine Shiga Toxin-ProducingToxins · 2020Article
- Assessing the genomic relatedness and evolutionary rates of persistent verotoxigenicMicrobial genomics · 2020Article
- The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-ProducingAnimals : an open access journal from MDPI · 2019Article
- Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-ProducingMicroorganisms · 2018Article
- Variability in CharacterizingMicroorganisms · 2018Article
- Genetic makeup of Shiga toxin-producing Escherichia coli in relation to clinical symptoms and duration of shedding: a microarray analysis of isolates from Swedish children.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Evidence for Contemporary Switching of the O-Antigen Gene Cluster between Shiga Toxin-ProducingFrontiers in microbiology · 2017Article
- Comparison of clinical and immunological findings in gnotobiotic piglets infected withGut pathogens · 2017Article
- The Accessory Genome of Shiga Toxin-Producing Escherichia coli Defines a Persistent Colonization Type in Cattle.Applied and environmental microbiology · 2016Article
- Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples.Frontiers in cellular and infection microbiology · 2015Article
- Comprehensive Characterization of Escherichia coli O104:H4 Isolated from Patients in the Netherlands.Frontiers in microbiology · 2015Article
- Development of a rapid microarray-based DNA subtyping assay for the alleles of Shiga toxins 1 and 2 of Escherichia coli.Journal of clinical microbiology · 2014Article
- Occurrence of Enterobacteriaceae in Raw Meat and in Human Samples from Egyptian Retail Sellers.International scholarly research notices · 2014Article
- The PathoChip, a functional gene array for assessing pathogenic properties of diverse microbial communities.The ISME journal · 2013Article
- Shiga toxin-producing Escherichia coli: factors involved in virulence and cattle colonization.Virulence · 2013Review
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6 authors.
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Abstract
Since enterohemorrhagic Escherichia coli (EHEC) isolates of serogroup O156 have been obtained from human diarrhea patients and asymptomatic carriers, we studied cattle as a potential reservoir for these bacteria. E. coli isolates serotyped by agglutination as O156:H25/H-/Hnt strains (n = 32) were isolated from three cattle farms during a period of 21 months and characterized by rapid microarray-based genotyping. The serotyping by agglutination of the O156 isolates was not confirmed in some cases by the results of DNA-based serotyping as only 25 of the 32 isolates were conclusively identified as O156:H25. In the multilocus sequence typing (MLST) analysis, all EHEC O156:H25 isolates were characterized as sequence type 300 (ST300) and ST688, which differ by a single-nucleotide exchange in the purA gene. Oligonucleotide microarrays allow simultaneous detection of a wider range of EHEC-associated and other E. coli virulence markers than other methods. All O156:H25 isolates showed a wide spectrum of virulence factors typical for EHEC. The stx(1) genes combined with the EHEC hlyA (hlyA(EHEC)) gene, the eae gene of the zeta subtype, as well as numerous other virulence markers were present in all EHEC O156:H25 strains. The behavior of eight different cluster groups, including four that were EHEC O156:H25, was monitored in space and time. Variations in the O156 cluster groups were detected. The results of the cluster analysis suggest that some O156:H25 strains had the genetic potential for a long persistence in the host and on the farm, while other strains did not. As judged by their pattern of virulence markers, E. coli O156:H25 isolates of bovine origin may represent a considerable risk for human infection. Our results showed that the miniaturized E. coli oligonucleotide arrays are an excellent tool for the rapid detection of a large number of virulence markers.
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