ArticleJournal of immunology (Baltimore, Md. : 1950)2010
Phosphorylation of leukocyte PECAM and its association with detergent-resistant membranes regulate transendothelial migration.
Article in Journal of immunology (Baltimore, Md. : 1950), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- CD13 activation assembles phosphoinositide (PI) signaling complexes to regulate the actin cytoskeleton.Scientific reports · 2026Article
- L-selectin regulates human neutrophil transendothelial migration.Journal of cell science · 2021Article
- Serine Phosphorylation of L-Selectin Regulates ERM Binding, Clustering, and Monocyte Protrusion in Transendothelial Migration.Frontiers in immunology · 2019Article
- Transendothelial migration: unifying principles from the endothelial perspective.Immunological reviews · 2016Review
- Targeted Recycling of the Lateral Border Recycling Compartment Precedes Adherens Junction Dissociation during Transendothelial Migration.The American journal of pathology · 2016Article
- The adhesion molecule PECAM-1 enhances the TGF-β-mediated inhibition of T cell function.Science signaling · 2016Article
- The regulation of transendothelial migration: new knowledge and new questions.Cardiovascular research · 2015Review
- Ecto-protein kinases and phosphatases: an emerging field for translational medicine.Journal of translational medicine · 2014Article
- Neutrophil and monocyte recruitment by PECAM, CD99, and other molecules via the LBRC.Seminars in immunopathology · 2014Review
- Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Article
- The CD157-integrin partnership controls transendothelial migration and adhesion of human monocytes.The Journal of biological chemistry · 2011Article
- Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity.Journal of cell science · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Leukocyte migration across the endothelial lining is a critical step in the body's response to infection and inflammation. The homophilic interaction between endothelial PECAM and leukocyte PECAM is essential for this process. The molecular events that are triggered in the endothelial cell by PECAM engagement have been well characterized; however, the function of leukocyte PECAM remains to be elucidated. To study this, we first blocked leukocyte transmigration using anti-PECAM Ab and then specifically activated leukocyte PECAM. This was sufficient to overcome the block and promote transmigration, suggesting an active signaling role for leukocyte PECAM. Consistent with this, we found that ligation of leukocyte PECAM induces phosphorylation of two tyrosine residues on its cytoplasmic tail. By performing RNA interference-rescue experiments, we demonstrate that these phosphorylation events are indispensable for transendothelial migration. Finally, we show that leukocyte PECAM translocates to a detergent-resistant membrane (DRM) during transmigration. PECAM localized in DRMs displays reduced phosphorylation and does not support transmigration. Together, these data support a model whereby engagement of leukocyte PECAM induces its transient tyrosine phosphorylation and induction of downstream signals that drive transmigration. These signals are then downregulated following PECAM translocation to DRMs.
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