ArticleNucleic acids research2010
All-codon scanning identifies p53 cancer rescue mutations.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- Oncogenic p53 induces mitotic errors in lung cancer cells by recopying DNA replication forks conferring targetable proliferation advantage.Cell death and differentiation · 2026Article
- Pyrimidine Triones as Potential Activators of p53 Mutants.Biomolecules · 2024Article
- Small-molecule correctors and stabilizers to target p53.Trends in pharmacological sciences · 2023Review
- High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell.PLoS pathogens · 2023Article
- ACE2 decoy receptor generated by high-throughput saturation mutagenesis efficiently neutralizes SARS-CoV-2 and its prevalent variants.Emerging microbes & infections · 2022Article
- Discovery of compounds that reactivate p53 mutants in vitro and in vivo.Cell chemical biology · 2022Article
- Targeting mutant p53 for efficient cancer therapy.Nature reviews. Cancer · 2018Review
- In silico identification of rescue sites by double force scanning.Bioinformatics (Oxford, England) · 2018Article
- Economical analysis of saturation mutagenesis experiments.Scientific reports · 2015Article
- CHOPER filters enable rare mutation detection in complex mutagenesis populations by next-generation sequencing.PloS one · 2015Article
- Contemporary, yeast-based approaches to understanding human genetic variation.Current opinion in genetics & development · 2013Review
- Article
- Computational identification of a transiently open L1/S3 pocket for reactivation of mutant p53.Nature communications · 2013Article
- Microbial synthetic biology for human therapeutics.Systems and synthetic biology · 2012Article
- Ensemble-based computational approach discriminates functional activity of p53 cancer and rescue mutants.PLoS computational biology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In vitro scanning mutagenesis strategies are valuable tools to identify critical residues in proteins and to generate proteins with modified properties. We describe the fast and simple All-Codon Scanning (ACS) strategy that creates a defined gene library wherein each individual codon within a specific target region is changed into all possible codons with only a single codon change per mutagenesis product. ACS is based on a multiplexed overlapping mutagenesis primer design that saturates only the targeted gene region with single codon changes. We have used ACS to produce single amino-acid changes in small and large regions of the human tumor suppressor protein p53 to identify single amino-acid substitutions that can restore activity to inactive p53 found in human cancers. Single-tube reactions were used to saturate defined 30-nt regions with all possible codon changes. The same technique was used in 20 parallel reactions to scan the 600-bp fragment encoding the entire p53 core domain. Identification of several novel p53 cancer rescue mutations demonstrated the utility of the ACS approach. ACS is a fast, simple and versatile method, which is useful for protein structure-function analyses and protein design or evolution problems.
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Registered trials
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