ArticleThe Journal of biological chemistry2010
The interplay of proton, electron, and metabolite supply for photosynthetic H2 production in Chlamydomonas reinhardtii.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 74 citations in OpenAlex.
- Metabolomic response to high light from pgrl1 and pgr5 mutants of Chlamydomonas reinhardtii.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Amino Acid Metabolic Vulnerabilities in Acute and Chronic Myeloid Leukemias.Frontiers in oncology · 2021Review
- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Metabolite Profiling and Network Analysis Reveal Coordinated Changes in Low-N Tolerant and Low-N Sensitive Maize Genotypes under Nitrogen Deficiency and Restoration Conditions.Plants (Basel, Switzerland) · 2020Article
- Coherent Point Drift Peak Alignment Algorithms Using Distance and Similarity Measures for Two-Dimensional Gas Chromatography Mass Spectrometry Data.Journal of chemometrics · 2020Article
- Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2019Article
- Subcellular Energetics and Carbon Storage inCells · 2019Review
- Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation.PloS one · 2018Article
- Microalgal hydrogen production: prospects of an essential technology for a clean and sustainable energy economy.Photosynthesis research · 2017Review
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Knock-Down of the IFR1 Protein Perturbs the Homeostasis of Reactive Electrophile Species and Boosts Photosynthetic Hydrogen Production inFrontiers in plant science · 2017Article
- Relevance of nutrient media composition for hydrogen production in Chlamydomonas.Photosynthesis research · 2015Review
- Article
- Engineering photosynthetic organisms for the production of biohydrogen.Photosynthesis research · 2015Review
- Article
- Copper response regulator1-dependent and -independent responses of the Chlamydomonas reinhardtii transcriptome to dark anoxia.The Plant cell · 2013Article
- De novo transcriptomic analysis of hydrogen production in the green alga Chlamydomonas moewusii through RNA-Seq.Biotechnology for biofuels · 2013Article
- Cellulose degradation and assimilation by the unicellular phototrophic eukaryote Chlamydomonas reinhardtii.Nature communications · 2012Article
- Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii.BMC genomics · 2011Article
- Time-course global expression profiles of Chlamydomonas reinhardtii during photo-biological H₂ production.PloS one · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To obtain a detailed picture of sulfur deprivation-induced H(2) production in microalgae, metabolome analyses were performed during key time points of the anaerobic H(2) production process of Chlamydomonas reinhardtii. Analyses were performed using gas chromatography coupled to mass spectrometry (GC/MS), two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GCxGC-TOFMS), lipid and starch analysis, and enzymatic determination of fermentative products. The studies were designed to provide a detailed metabolite profile of the solar Bio-H(2) production process. This work reports on the differential analysis of metabolic profiles of the high H(2)-producing strain Stm6Glc4 and the wild-type cc406 (WT) before and during the H(2) production phase. Using GCxGC-TOFMS analysis the number of detected peaks increased from 128 peaks, previously detected by GC/MS techniques, to ∼1168. More detailed analysis of the anaerobic H(2) production phase revealed remarkable differences between wild-type and mutant cells in a number of metabolic pathways. Under these physiological conditions the WT produced up to 2.6 times more fatty acids, 2.2 times more neutral lipids, and up to 4 times more fermentation products compared with Stm6Glc4. Based on these results, specific metabolic pathways involving the synthesis of fatty acids, neutral lipids, and fermentation products during anaerobiosis in C. reinhardtii have been identified as potential targets for metabolic engineering to further enhance substrate supply for the hydrogenase(s) in the chloroplast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.