Evidence map›Paper›PMID 20580973›Full record

ArticleMethods in enzymology2010

Single mRNA tracking in live cells.

Hye Yoon Park, Adina R Buxbaum, Robert H Singer

Abstract read
In one paragraph

Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. LNCcation: lncRNA localization and function.The Journal of cell biology · 2021
    Review
  6. Article
  7. Article
  8. Review
  9. A time-varying approach to single particle tracking with a nonlinear observation model.Proceedings of the ... American Control Conference. American Control Conference · 2020
    Article
  10. Article
  11. Review
  12. CRISPR Tools for Systematic Studies of RNA Regulation.Cold Spring Harbor perspectives in biology · 2019
    Review
  13. Article
  14. Quantifying Single mRNA Translation Kinetics in Living Cells.Cold Spring Harbor perspectives in biology · 2018
    Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hye Yoon ParkAnatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, USA.
Adina R Buxbaum
Robert H Singer

Funding

LIGHT-ACTIVATED GENE EXPRESSION IN SINGLE CELLS WITHIN TISSUER01GM086217 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI SINGER, ROBERT H · 2008 to 2011
$2.9M
MECHANISMS OF CARDIOVASCULAR DISEASEST32HL007675 · NHLBI · YESHIVA UNIVERSITY · PI SIBINGA, NICHOLAS E · 1989 to 2013
$2.2M
SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLSR01EB002060 · NIBIB · YESHIVA UNIVERSITY · PI SINGER, ROBERT H · 2003 to 2008
$2.0M
Mechanism of Actin mRNA LocalizationR01GM084364 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI SINGER, ROBERT H · 2008 to 2012
$1.9M
Observation of Single mRNA Molecules in Living Cells in TissueF32GM087122 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI PARK, HYE YOON · 2009 to 2011
$108k
NHLBI NIH HHS 5T32 HL007675NHLBI NIH HHS T32 HL007675NIBIB NIH HHS EB2060NIBIB NIH HHS R01 EB002060NIGMS NIH HHS 1F32 GM087122NIGMS NIH HHS F32 GM087122NIGMS NIH HHS R01 GM084364NIGMS NIH HHS R01 GM086217
6 · The paper itself

Abstract

Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.

Indexed as

AlgorithmsAnimalsFluorescent DyesImage Processing, Computer-AssistedMicroscopy, FluorescenceRecombinant Fusion ProteinsRNA, MessengerRNA TransportFluorescent DyesRecombinant Fusion ProteinsRNA, Messenger

Identifiers

PMID20580973
PMCPMC3158948

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.