ArticleMethods in enzymology2010
Watching individual proteins acting on single molecules of DNA.
Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae.Nucleic acids research · 2023Article
- BRCA2 chaperones RAD51 to single molecules of RPA-coated ssDNA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies.Journal of visualized experiments : JoVE · 2022Article
- Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins.Molecular cell · 2021Article
- Observation and Analysis of RAD51 Nucleation Dynamics at Single-Monomer Resolution.Methods in enzymology · 2018Article
- Article
- RecA: Regulation and Mechanism of a Molecular Search Engine.Trends in biochemical sciences · 2016Review
- Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases.Methods in enzymology · 2016Article
- Single-molecule visualization of RecQ helicase reveals DNA melting, nucleation, and assembly are required for processive DNA unwinding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Characterizing the interaction between DNA and GelRed fluorescent stain.European biophysics journal : EBJ · 2015Article
- DNA unwinding heterogeneity by RecBCD results from static molecules able to equilibrate.Nature · 2013Article
- Fluorescent labeling and modification of proteins.Journal of chemical biology · 2013Review
- Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA dumbbells with optical traps, microfluidics and fluorescence microscopy.Nature protocols · 2013Article
- Optical Methods to Study Protein-DNA Interactions in Vitro and in Living Cells at the Single-Molecule Level.International journal of molecular sciences · 2013Article
- Alteration of χ recognition by RecBCD reveals a regulated molecular latch and suggests a channel-bypass mechanism for biological control.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Dynamics of lesion processing by bacterial nucleotide excision repair proteins.Progress in molecular biology and translational science · 2012Review
- Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications.Genes & development · 2011Review
- Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.Trends in cell biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In traditional biochemical experiments, the behavior of individual proteins is obscured by ensemble averaging. To better understand the behavior of proteins that bind to and/or translocate on DNA, we have developed instrumentation that uses optical trapping, microfluidic solution delivery, and fluorescent microscopy to visualize either individual proteins or assemblies of proteins acting on single molecules of DNA. The general experimental design involves attaching a single DNA molecule to a polystyrene microsphere that is then used as a microscopic handle to manipulate individual DNA molecules with a laser trap. Visualization is achieved by fluorescently labeling either the DNA or the protein of interest, followed by direct imaging using high-sensitivity fluorescence microscopy. We describe the sample preparation and instrumentation used to visualize the interaction of individual proteins with single molecules of DNA. As examples, we describe the application of these methods to the study of proteins involved in recombination-mediated DNA repair, a process essential for the maintenance of genomic integrity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.