ArticleVirus genes1991
Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.
Article in Virus genes, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 12 citations in OpenAlex.
- The impact of hepatitis B virus x protein and microRNAs in hepatocellular carcinoma: a comprehensive analysis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Review
- The viral transactivator HBx protein exhibits a high potential for regulation via phosphorylation through an evolutionarily conserved mechanism.Infectious agents and cancer · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hepatocellular carcinoma contains an X ORF and a 5' extended preX/X ORF, which were both used to construct X recombinant baculoviruses. Infection of Sf9 insect cells with these recombinant viruses yielded large amounts of the respective X proteins. They were identified by a set of mouse monoclonal antibodies directed against different epitopes of the ayw X protein using immunoblotting techniques. A subpopulation of the X protein expressed is modified, thus raising the molecular weight from the expected size of 17 kD to 21 kD. Indirect immunofluorescence and immunoelectron microscopy was performed to characterize the subcellular distribution of the X protein expressed in Sf9 cells. Data are presented that it accumulates as large globular structures within the cytoplasm and the nucleus of the infected cells.
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Registered trials
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