ArticleJournal of molecular biology2010
Discoidin I from Dictyostelium discoideum and Interactions with oligosaccharides: specificity, affinity, crystal structures, and comparison with discoidin II.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- F-type lectins: Structural and functional aspects, and potential biomedical applications.BBA advances · 2025Article
- Exploring host-pathogen interactions in the Dictyostelium discoideum-Mycobacterium marinum infection model of tuberculosis.Disease models & mechanisms · 2024Review
- Role of Natural Binding Proteins in Therapy and Diagnostics.Life (Basel, Switzerland) · 2024Article
- Unravelling mechanisms of bacterial recognition by Acanthamoeba: insights into microbial ecology and immune responses.Frontiers in microbiology · 2024Review
- Exploring the Cellular and Molecular Mechanism of Discoidin Domain Receptors (DDR1 and DDR2) in Bone Formation, Regeneration, and Its Associated Disease Conditions.International journal of molecular sciences · 2023Review
- The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery.The Journal of cell biology · 2022Article
- First Insights into the Repertoire of Secretory Lectins in Rotifers.Marine drugs · 2022Article
- High-Throughput Generation of Product Profiles for Arabinoxylan-Active Enzymes from Metagenomes.Applied and environmental microbiology · 2020Article
- Discoidin domain receptors: Micro insights into macro assemblies.Biochimica et biophysica acta. Molecular cell research · 2019Review
- Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenicProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin.Molecular biology of the cell · 2018Article
- The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence.Scientific reports · 2017Article
- Eat Prey, Live:Frontiers in immunology · 2017Review
- F-Type Lectins: A Highly Diversified Family of Fucose-Binding Proteins with a Unique Sequence Motif and Structural Fold, Involved in Self/Non-Self-Recognition.Frontiers in immunology · 2017Review
- Cooperative cell motility during tandem locomotion of amoeboid cells.Molecular biology of the cell · 2016Article
- The first identification of carbohydrate binding modules specific to chitosan.The Journal of biological chemistry · 2013Article
- X-linked juvenile retinoschisis: clinical diagnosis, genetic analysis, and molecular mechanisms.Progress in retinal and eye research · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Discoidin I (DiscI) and discoidin II (DiscII) are N-acetylgalactosamine (GalNAc)-binding proteins from Dictyostelium discoideum. They consist of two domains: an N-terminal discoidin domain and a C-terminal H-type lectin domain. They were cloned and expressed in high yield in recombinant form in Escherichia coli. Although both lectins bind galactose (Gal) and GalNAc, glycan array experiments performed on the recombinant proteins displayed strong differences in their specificity for oligosaccharides. DiscI and DiscII bind preferentially to Gal/GalNAcbeta1-3Gal/GalNAc-containing and Gal/GalNAcbeta1-4GlcNAcbeta1-6Gal/GalNAc-containing glycans, respectively. The affinity of the interaction of DiscI with monosaccharides and disaccharides was evaluated using isothermal titration calorimetry experiments. The three-dimensional structures of native DiscI and its complexes with GalNAc, GalNAcbeta1-3Gal, and Galbeta1-3GalNAc were solved by X-ray crystallography. DiscI forms trimers with involvement of calcium at the monomer interface. The N-terminal discoidin domain presents a structural similarity to F-type lectins such as the eel agglutinin, where an amphiphilic binding pocket suggests possible carbohydrate-binding activity. In the C-terminal H-type lectin domain, the GalNAc residue establishes specific hydrogen bonds that explain the observed affinity (K(d)=3x10(-4) M). The different specificities of DiscI and DiscII for oligosaccharides were rationalized from the different structures obtained by either X-ray crystallography or molecular modeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.