ArticleIn vitro cellular & developmental biology. Animal2010
Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790).
Article in In vitro cellular & developmental biology. Animal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi).Archives of virology · 2024Article
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus).In vitro cellular & developmental biology. Animal · 2021Article
- Establishment and characterization of a cell line derived from fin of the endangered Yangtze sturgeon (Acipenser dabryanus).In vitro cellular & developmental biology. Animal · 2020Article
- Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar, Astronotus ocellatus.Fish physiology and biochemistry · 2019Article
- A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864).Cytotechnology · 2016Article
- Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793).In vitro cellular & developmental biology. Animal · 2015Article
- Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India.In vitro cellular & developmental biology. Animal · 2015Article
- Development and characterization of a cell line WAF from freshwater shark Wallago attu.Molecular biology reports · 2014Article
- Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.Fish physiology and biochemistry · 2013Article
- Development and characterization of a new cell line CF from caudal fin of knifefish, Chitala chitala (Hamilton-Buchanan).In vitro cellular & developmental biology. Animal · 2013Article
- Establishment and characterization of two fetal fibroblast cell lines from the yak.In vitro cellular & developmental biology. Animal · 2012Article
- Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2012Article
- Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.).Fish physiology and biochemistry · 2012Article
- Establishment of a macrophage cell line from adherent peripheral blood mononuclear cells of Catla catla.In vitro cellular & developmental biology. Animal · 2012Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new cell line [pearlspot fin (PSF)] has been developed from caudal fin of Etroplus suratensis, a brackish/freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum (FBS). The PSF cell line consisted predominantly of epithelial-like cells. The cells were able to grow at temperatures between 25 degrees C and 32 degrees C with optimum temperature of 28 degrees C. The growth rate of PSF cells increased as the FBS proportion increased from 2% to 20% at 28 degrees C with optimum growth at the concentration of 10% FBS. One marine fish virus (fish nodavirus) was tested on this cell line and found not susceptible. After confluency, the cells were subcultured with a split ratio of 1:2. The cells showed epithelial-like morphology and reached confluency on the third d after subculture. Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI indicated identity of this cell line with those reported from this fish species, confirming that the cell line was of pearlspot origin. The cells were successfully cryopreserved and revived at the tenth, 25th, and 35th passages. The bacterial extracellular products from Vibrio cholerae MTCC 3904 were found to be toxic to PSF. Karyotyping analysis indicated that the modal chromosome number was 48.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.